Print Email Facebook Twitter Automated spherical aberration correction in scanning confocal microscopy Title Automated spherical aberration correction in scanning confocal microscopy Author Yoo, H.W. Royen, M.E. van Cappellen, W.A. Houtsmuller, A.B. Verhaegen, M.H.G. Schitter, G. Faculty Mechanical, Maritime and Materials Engineering Department Delft Center for Systems and Control Date 2014-12-18 Abstract Mismatch between the refractive indexes of immersion media and glass coverslips introduces spherical aberrations in microscopes especially for high numerical aperture objectives. This contribution demonstrates an automated adjustment of the coverslip correction collar in scanning confocal microscopy to compensate for spherical aberrations due to coverslip thickness mismatch. With a motorized coverslip correction collar, the adjustment procedure consists of xz image scans, image processing, correction quality evaluation, the mismatch estimation, and eventually the optimal adjustment of the correction collar. For fast correction with less photodamage, coarse-fine Gaussian fitting algorithms are proposed and evaluated with various specimen for their estimation accuracy. The benefits of the proposed automated correction are demonstrated for various coverslips with biological specimens, showing the optimized resolution of the confocal microscope. Subject mage sensorsspherical aberrationsmedical imagingconfocal microscopesentropy To reference this document use: http://resolver.tudelft.nl/uuid:026751c2-14b6-4b19-b3c5-f70ca59e091e DOI https://doi.org/10.1063/1.4904370 Publisher American Institute of Physics ISSN 0034-6748 Source http://scitation.aip.org/content/aip/journal/rsi/85/12/10.1063/1.4904370 Source Review of Scientific Instruments, 85 (12), 2014 Part of collection Institutional Repository Document type journal article Rights © 2014 AIP Publishing Files PDF 312912.pdf 1.15 MB Close viewer /islandora/object/uuid:026751c2-14b6-4b19-b3c5-f70ca59e091e/datastream/OBJ/view