Print Email Facebook Twitter Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips Title Quantitative chemical biosensing by bacterial chemotaxis in microfluidic chips Author Roggo, Clémence (University of Lausanne) Picioreanu, C. (TU Delft BT/Environmental Biotechnology) Richard, Xavier (University of Fribourg) Mazza, Christian (University of Fribourg) van Lintel, Harald (Swiss Federal Institute of Technology) van der Meer, Jan Roelof (University of Lausanne) Date 2018-01-01 Abstract Whole-cell bacterial bioreporters are proposed as alternatives to chemical analysis of, for example, pollutants in environmental compartments. Commonly based on reporter gene induction, bioreporters produce a detectable signal within 30 min to a few hours after exposure to the chemical target, which is impractical for applications aiming at a fast response. In an attempt to attain faster readout but maintain flexibility of chemical targeting, we explored the concept for quantitative chemical sensing by bacterial chemotaxis. Chemotaxis was quantified from enrichment of cells across a 600 µm-wide chemical gradient stabilized by parallel flow in a microfluidic chip, further supported by transport and chemotaxis steady state and kinetic modelling. As proof-of-concept, we quantified Escherichia coli chemotaxis towards serine, aspartate and methylaspartate as a function of attractant concentration and exposure time. E. coli chemotaxis enrichment increased sharply between 0 and 10 µM serine, before saturating at 100 µM. The chemotaxis accumulation rate was maximal at 10 µM serine, leading to observable cell enrichment within 5 min. The potential application for biosensing of environmental toxicants was investigated by quantifying chemotaxis of Cupriavidus pinatubonensis JMP134 towards the herbicide 2,4-dichlorophenoxyacetate. Our results show that bacterial chemotaxis can be quantified on a scale of minutes and may be used for developing faster bioreporter assays. To reference this document use: http://resolver.tudelft.nl/uuid:036b92ec-9f1d-4981-b283-178678a40924 DOI https://doi.org/10.1111/1462-2920.13982 ISSN 1462-2912 Source Environmental Microbiology, 20 (1), 241-258 Part of collection Institutional Repository Document type journal article Rights © 2018 Clémence Roggo, C. Picioreanu, Xavier Richard, Christian Mazza, Harald van Lintel, Jan Roelof van der Meer Files PDF Roggo_et_al_2018_Environm ... iology.pdf 1.67 MB Close viewer /islandora/object/uuid:036b92ec-9f1d-4981-b283-178678a40924/datastream/OBJ/view