Print Email Facebook Twitter Mapping of Enzyme Kinetics on a Microfluidic Device Title Mapping of Enzyme Kinetics on a Microfluidic Device Author Rho, Hoon Suk (University of Twente) Hanke, A.T. (TU Delft BT/Bioprocess Engineering) Ottens, M. (TU Delft BT/Bioprocess Engineering) Gardeniers, Han (University of Twente) Date 2016 Abstract A microfluidic platform or "microfluidic mapper" is demonstrated, which in a single experiment performs 36 parallel biochemical reactions with 36 different combinations of two reagents in stepwise concentration gradients. The volume used in each individual reaction was 36 nl. With the microfluidic mapper, we obtained a 3D enzyme reaction plot of horseradish peroxidase (HRP) with Amplex Red (AR) and hydrogen peroxide (H2O2), for concentration ranges of 11.7 μM to 100.0 μM and 11.1 μM to 66.7 μM for AR and H2O2, respectively. This system and methodology could be used as a fast analytical tool to evaluate various chemical and biochemical reactions especially where two or more reagents interact with each other. The generation of dual concentration gradients in the present format has many advantages such as parallelization of reactions in a nanoliter-scale volume and the real-time monitoring of processes leading to quick concentration gradients. The microfluidic mapper could be applied to various problems in analytical chemistry such as revealing of binding kinetics, and optimization of reaction kinetics. To reference this document use: http://resolver.tudelft.nl/uuid:039599f1-7412-495a-92d4-96d913c31032 DOI https://doi.org/10.1371/journal.pone.0153437 ISSN 1932-6203 Source PLoS ONE, 11 (4), e0153437 Part of collection Institutional Repository Document type journal article Rights © 2016 Hoon Suk Rho, A.T. Hanke, M. Ottens, Han Gardeniers Files PDF journal.pone.0153437.PDF 2.87 MB Close viewer /islandora/object/uuid:039599f1-7412-495a-92d4-96d913c31032/datastream/OBJ/view