Old yellow enzyme-catalysed asymmetric hydrogenation

Old yellow enzyme-catalysed asymmetric hydrogenation: Linking Family Roots with Improved Catalysis

Scholtissek, Anika (University of Technology Bergakademie Freiberg)
Tischler, Dirk (University of Technology Bergakademie Freiberg)
Westphal, Adrie H. (Wageningen University & Research)
van Berkel, Willem J.H. (Wageningen University & Research)
Paul, C.E. (TU Delft BT/Biocatalysis)

2017

Asymmetric hydrogenation of activated alkenes catalysed by ene-reductases from the old yellow enzyme family (OYEs) leading to chiral products is of potential interest for industrial processes. OYEs’ dependency on the pyridine nucleotide coenzyme can be circumvented through established artificial hydride donors such as nicotinamide coenzyme biomimetics (NCBs). Several OYEs were found to exhibit higher reduction rates with NCBs. In this review, we describe a new classification of OYEs into three main classes by phylogenetic and structural analysis of characterized OYEs. The family roots are linked with their use as chiral catalysts and their mode of action with NCBs. The link between bioinformatics (sequence analysis), biochemistry (structure–function analysis), and biocatalysis (conversion, enantioselectivity and kinetics) can enable an early classification of a putative ene-reductase and therefore the indication of the binding mode of various activated alkenes

old yellow enzymes
nicotinamide coenzyme biomimetics
cofactor analogues
classification of OYE
oxidoreductases
asymmetric hydrogenation
selective reduction
phylogenetics
OA-Fund TU Delft

http://resolver.tudelft.nl/uuid:049993ba-e449-4579-878b-32a345939639

https://doi.org/10.3390/catal7050130

2073-4344

Catalysts, 7 (5)

Institutional Repository

journal article

© 2017 Anika Scholtissek, Dirk Tischler, Adrie H. Westphal, Willem J.H. van Berkel, C.E. Paul