Print Email Facebook Twitter Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading Title Analysis of ammonia-oxidizing bacteria dominating in lab-scale bioreactors with high ammonium bicarbonate loading Author Vejmelkova, D. Sorokin, D.Y. Abbas, B. Kovaleva, O.L. Kleerebezem, R. Kampschreur, M.J. Muyzer, G. Van Loosdrecht, M.C.M. Faculty Applied Sciences Department Biotechnology Date 2011-06-21 Abstract The ammonia-oxidizing bacterial community (AOB) was investigated in two types of laboratory-scale bioreactors performing partial oxidation of ammonia to nitrite or nitrate at high (80 mM) to extremely high (428 mM) concentrations of ammonium bicarbonate. At all conditions, the dominant AOB was affiliated to the Nitrosomonas europaea lineage as was determined by fluorescence in situ hybridization and polymerase chain reaction in combination with denaturing gradient gel electrophoresis. Molecular analysis of the mixed populations, based on the 16S rRNA and cbbL genes, demonstrated the presence of two different phylotypes of Nitrosomonas, while microbiological analysis produced a single phylotype, represented by three different morphotypes. One of the most striking features of the AOB populations encountered in the bioreactors was the domination of highly aggregated obligate microaerophilic Nitrosomonas, with unusual cellular and colony morphology, commonly observed in nitrifying bioreactors but rarely investigated by cultural methods. The latter is probably not an adaptation to stressful conditions created by high ammonia or nitrite concentrations, but oxygen seems to be a stressful factor in these bioreactors. Subject ammonia-oxidizing bacteria (AOB)DGGEFISHNitrosomonaspartial nitrification To reference this document use: http://resolver.tudelft.nl/uuid:05233356-d72a-45d3-98e8-9cd76a1638b4 DOI https://doi.org/10.1007/s00253-011-3409-x Publisher Springer-Verlag ISSN 0175-7598 Source http://www.springerlink.com/content/c2qv4707h6t50777/ Source Applied Microbiology and Biotechnology, 93 (1), 2011 Part of collection Institutional Repository Document type journal article Rights (c) 2011 Springer-Verlag Files PDF vejmelkova.pdf 653.61 KB Close viewer /islandora/object/uuid:05233356-d72a-45d3-98e8-9cd76a1638b4/datastream/OBJ/view