Print Email Facebook Twitter CRISPR-Cas Title CRISPR-Cas: Adapting to change Author Jackson, Simon A. (University of Otago) McKenzie, R. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Fagerlund, Robert D. (University of Otago) Kieper, S.N. (TU Delft BN/Stan Brouns Lab; Kavli institute of nanoscience Delft) Fineran, Peter C. (University of Otago) Brouns, S.J.J. (TU Delft BN/Stan Brouns Lab; Wageningen University & Research; Kavli institute of nanoscience Delft) Date 2017-04-07 Abstract Bacteria and archaea are engaged in a constant arms race to defend against the ever-present threats of viruses and invasion by mobile genetic elements. The most flexible weapons in the prokaryotic defense arsenal are the CRISPR-Cas adaptive immune systems. These systems are capable of selective identification and neutralization of foreign DNA and/or RNA. CRISPR-Cas systems rely on stored genetic memories to facilitate target recognition. Thus, to keep pace with a changing pool of hostile invaders, the CRISPR memory banks must be regularly updated with new information through a process termed CRISPR adaptation. In this Review, we outline the recent advances in our understanding of the molecular mechanisms governing CRISPR adaptation. Specifically, the conserved protein machinery Cas1-Cas2 is the cornerstone of adaptive immunity in a range of diverse CRISPR-Cas systems. To reference this document use: http://resolver.tudelft.nl/uuid:0baaa190-8e07-4c91-9114-1bd0cc430238 DOI https://doi.org/10.1126/science.aal5056 ISSN 0036-8075 Source Science, 356 (6333), 1-9 Part of collection Institutional Repository Document type journal article Rights © 2017 Simon A. Jackson, R. McKenzie, Robert D. Fagerlund, S.N. Kieper, Peter C. Fineran, S.J.J. Brouns Files PDF Jackson_et_al_Science_201 ... n_Text.pdf 4.42 MB Close viewer /islandora/object/uuid:0baaa190-8e07-4c91-9114-1bd0cc430238/datastream/OBJ/view