Print Email Facebook Twitter Light-sheet optimization for microscopy Title Light-sheet optimization for microscopy Author Wilding, D. (TU Delft Team Raf Van de Plas) Pozzi, P. (TU Delft Team Raf Van de Plas) Soloviev, O.A. (TU Delft Team Raf Van de Plas; Flexible Optical B.V.) Vdovin, Gleb (TU Delft Team Raf Van de Plas; Flexible Optical B.V.) Verhaegen, M.H.G. (TU Delft Team Raf Van de Plas) Contributor Bifano, Thomas G. (editor) Kubby, Joel (editor) Gigan, Sylvain (editor) Date 2016 Abstract Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is standard selective plane illumination microscope (SPIM) configuration modified with the addition of a spatial light modulator (SLM) and a third objective for the detection of transmitted light. Optimization protocols use this transmission light allowing the extension the depth-of-field and correction of aberrations whilst retaining a thin optical section. Subject Adaptive opticsimagingmicroscopylight-sheet microscopyoptimization To reference this document use: http://resolver.tudelft.nl/uuid:0d3a0695-3eb6-4da3-a341-e022df2c8629 DOI https://doi.org/10.1117/12.2210912 Publisher SPIE, Bellingham, WA, USA ISBN 978-1-628419511 Source Proceeedings of SPIE: Adaptive Optics and Wavefront Control for Biological Systems II, 9717 Event Adaptive Optics and Wavefront Control for Biological Systems II, 2016-02-13 → 2016-02-15, San Francisco, United States Series Proceedings of SPIE, 1605-7422, 9717 Part of collection Institutional Repository Document type conference paper Rights © 2016 D. Wilding, P. Pozzi, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen Files PDF 971718.pdf 547.31 KB Close viewer /islandora/object/uuid:0d3a0695-3eb6-4da3-a341-e022df2c8629/datastream/OBJ/view