Print Email Facebook Twitter In vivo analysis of Saccharomyces cerevisiae plasma membrane ATPase Pma1p isoforms with increased in vitro H+/ATP stoichiometry Title In vivo analysis of Saccharomyces cerevisiae plasma membrane ATPase Pma1p isoforms with increased in vitro H+/ATP stoichiometry Author De Kok, S. Yilmaz, D. Daran, J.M. Pronk, J.T. Van Maris, A.J.A. Faculty Applied Sciences Department BT/Biotechnology Date 2012-04-10 Abstract Plasma membrane H+-ATPase isoforms with increased H+/ATP ratios represent a desirable asset in yeast metabolic engineering. In vivo proton coupling of two previously reported Pma1p isoforms (Ser800Ala, Glu803Gln) with increased in vitro H+/ATP stoichiometries was analysed by measuring biomass yields of anaerobic maltose-limited chemostat cultures expressing only the different PMA1 alleles. In vivo H+/ATP stoichiometries of wildtype Pma1p and the two isoforms did not differ significantly. Subject Pma1Ser800AlaGlu803GlnMaltoseYeastProton symport To reference this document use: http://resolver.tudelft.nl/uuid:106b1146-45ed-43a5-bafc-779358ecf9b6 DOI https://doi.org/10.1007/s10482-012-9730-2 Publisher Springer ISSN 0003-6072 Source http://www.springerlink.com/content/e155u00p2632l43m/ Source Antonie van Leeuwenhoek, 102 (2), 2012 Part of collection Institutional Repository Document type journal article Rights (c) 2012 The Author(s)This article is published with open access at Springerlink.com Files PDF vanMaris_2012.pdf 204.83 KB Close viewer /islandora/object/uuid:106b1146-45ed-43a5-bafc-779358ecf9b6/datastream/OBJ/view