Print Email Facebook Twitter The condensin complex is a mechanochemical motor that translocates along DNA Title The condensin complex is a mechanochemical motor that translocates along DNA Author Terakawa, Tsuyoshi (Columbia University) Bisht, Shveta (European Molecular Biology Laboratory Heidelberg) Eeftens, J.M. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Haering, Christian H. (European Molecular Biology Laboratory Heidelberg) Greene, Eric C. (Columbia University) Date 2017-11-03 Abstract Condensin plays crucial roles in chromosome organization and compaction, but the mechanistic basis for its functions remains obscure. We used single-molecule imaging to demonstrate that Saccharomyces cerevisiae condensin is a molecular motor capable of adenosine triphosphate hydrolysis–dependent translocation along double-stranded DNA. Condensin’s translocation activity is rapid and highly processive, with individual complexes traveling an average distance of ≥10 kilobases at a velocity of ~60 base pairs per second. Our results suggest that condensin may take steps comparable in length to its ~50-nanometer coiled-coil subunits, indicative of a translocation mechanism that is distinct from any reported for a DNA motor protein. The finding that condensin is a mechanochemical motor has important implications for understanding the mechanisms of chromosome organization and condensation. To reference this document use: http://resolver.tudelft.nl/uuid:153c569a-1bff-4ec0-8f6d-2a62981fcf35 DOI https://doi.org/10.1126/science.aan6516 ISSN 0036-8075 Source Science, 358 (6363), 672-676 Part of collection Institutional Repository Document type journal article Rights © 2017 Tsuyoshi Terakawa, Shveta Bisht, J.M. Eeftens, C. Dekker, Christian H. Haering, Eric C. Greene Files PDF Condensin_Science_051317.pdf 2.22 MB Close viewer /islandora/object/uuid:153c569a-1bff-4ec0-8f6d-2a62981fcf35/datastream/OBJ/view