Print Email Facebook Twitter Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points Title Human centromeric CENP-A chromatin is a homotypic, octameric nucleosome at all cell cycle points Author Nechemia-Arbely, Yael (University of California) Fachinetti, Daniele (University of California) Miga, Karen H. (University of California) Sekulic, Nikolina (University of Pennsylvania) Soni, G.V. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Kim, Dong Hyun (University of California) Wong, Adeline K. (University of California) Lee, Ah Young (University of California) Nguyen, Kristen (University of California) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Ren, Bing (University of California) Black, Ben E. (University of Pennsylvania) Cleveland, Don W. (University of California) Date 2017-03-06 Abstract Chromatin assembled with centromere protein A (CENP-A) is the epigenetic mark of centromere identity. Using new reference models, we now identify sites of CENP-A and histone H3.1 binding within the megabase, α-satellite repeat-containing centromeres of 23 human chromosomes. The overwhelming majority (97%) of α-satellite DNA is found to be assembled with histone H3.1-containing nucleosomes with wrapped DNA termini. In both G1 and G2 cell cycle phases, the 2-4% of α-satellite assembled with CENP-A protects DNA lengths centered on 133 bp, consistent with octameric nucleosomes with DNA unwrapping at entry and exit. CENP-A chromatin is shown to contain equimolar amounts of CENP-A and histones H2A, H2B, and H4, with no H3. Solid-state nanopore analyses show it to be nucleosomal in size. Thus, in contrast to models for hemisomes that briefly transition to octameric nucleosomes at specific cell cycle points or heterotypic nucleosomes containing both CENP-A and histone H3, human CENP-A chromatin complexes are octameric nucleosomes with two molecules of CENP-A at all cell cycle phases. To reference this document use: http://resolver.tudelft.nl/uuid:19164e31-0f3e-49d6-b128-3af464f3cfd2 DOI https://doi.org/10.1083/jcb.201608083 Embargo date 2017-08-24 ISSN 0021-9525 Source The Journal of Cell Biology, 216 (3), 607-621 Part of collection Institutional Repository Document type journal article Rights © 2017 Yael Nechemia-Arbely, Daniele Fachinetti, Karen H. Miga, Nikolina Sekulic, G.V. Soni, Dong Hyun Kim, Adeline K. Wong, Ah Young Lee, Kristen Nguyen, C. Dekker, Bing Ren, Ben E. Black, Don W. Cleveland Files PDF 607.full.pdf 2.54 MB Close viewer /islandora/object/uuid:19164e31-0f3e-49d6-b128-3af464f3cfd2/datastream/OBJ/view