Print Email Facebook Twitter Growth media in anaerobic fermentative processes Title Growth media in anaerobic fermentative processes: The underestimated potential of thermophilic fermentation and anaerobic digestion Author Hendriks, A.T.W.M. (TU Delft Sanitary Engineering) van Lier, J.B. (TU Delft Sanitary Engineering) de Kreuk, M.K. (TU Delft Sanitary Engineering) Date 2018 Abstract Fermentation and anaerobic digestion of organic waste and wastewater is broadly studied and applied. Despite widely available results and data for these processes, comparison of the generated results in literature is difficult. Not only due to the used variety of process conditions, but also because of the many different growth media that are used. Composition of growth media can influence biogas production (rates) and lead to process instability during anaerobic digestion. To be able to compare results of the different studies reported, and to ensure nutrient limitation is not influencing observations ascribed to process dynamics and/or reaction kinetics, a standard protocol for creating a defined growth medium for anaerobic digestion and mixed culture fermentation is proposed. This paper explains the role(s) of the different macro- and micronutrients, as well as the choices for a growth medium formulation strategy. In addition, the differences in nutrient requirements between mesophilic and thermophilic systems are discussed as well as the importance of specific trace metals regarding specific conversion routes and the possible supplementary requirement of vitamins. The paper will also give some insight into the bio-availability and toxicity of trace metals. A remarkable finding is that mesophilic and thermophilic enzymes are quite comparable at their optimum temperatures. This has consequences for the trace metal requirements of thermophiles under certain conditions. Under non-limiting conditions, the trace metal requirement of thermophilic systems is about 3 times higher than for mesophilic systems. Subject Anaerobic digestionBiogasEnzymeFermentationHydrogenMesophilicNutrientThermophilicTrace metalVolatile fatty acid To reference this document use: http://resolver.tudelft.nl/uuid:1a6c1a73-6ee9-4583-89b6-7a2c193adfd6 DOI https://doi.org/10.1016/j.biotechadv.2017.08.004 Embargo date 2018-10-07 ISSN 0734-9750 Source Biotechnology Advances, 36 (1), 1-13 Part of collection Institutional Repository Document type journal article Rights © 2018 A.T.W.M. Hendriks, J.B. van Lier, M.K. de Kreuk Files PDF Growth_media_in_anaerobic ... _Clean.pdf 908.29 KB Close viewer /islandora/object/uuid:1a6c1a73-6ee9-4583-89b6-7a2c193adfd6/datastream/OBJ/view