Print Email Facebook Twitter Visualization of unstained DNA nanostructures with advanced in-focus phase contrast TEM techniques Title Visualization of unstained DNA nanostructures with advanced in-focus phase contrast TEM techniques Author Kabiri, Y. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Ravelli, Raimond B.G. (Maastricht Multimodal Molecular Imaging Institute) Lehnert, Tibor (University of Ulm) Qi, Haoyuan (University of Ulm) Katan, A.J. (TU Delft QN/Afdelingsbureau; Kavli institute of nanoscience Delft) Roest, Natascha (Maastricht Multimodal Molecular Imaging Institute) Kaiser, Ute (University of Ulm) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Peters, Peter J. (Maastricht Multimodal Molecular Imaging Institute) Zandbergen, H.W. (TU Delft QN/Zandbergen Lab; Kavli institute of nanoscience Delft) Date 2019 Abstract Over the last few years, tremendous progress has been made in visualizing biologically important macromolecules using transmission electron microscopy (TEM) and understanding their structure-function relation. Yet, despite the importance of DNA in all forms of life, TEM visualization of individual DNA molecules in its native unlabeled form has remained extremely challenging. Here, we present high-contrast images of unstained single-layer DNA nanostructures that were obtained using advanced in-focus phase contrast TEM techniques. These include sub-Ångstrom low voltage electron microscopy (SALVE), the use of a volta-potential phase plate (VPP), and dark-field (DF) microscopy. We discuss the advantages and drawbacks of these techniques for broad applications in structural biology and materials science. To reference this document use: http://resolver.tudelft.nl/uuid:1b18711b-c3e0-430d-9701-05008b184588 DOI https://doi.org/10.1038/s41598-019-43687-5 ISSN 2045-2322 Source Scientific Reports, 9 (1) Part of collection Institutional Repository Document type journal article Rights © 2019 Y. Kabiri, Raimond B.G. Ravelli, Tibor Lehnert, Haoyuan Qi, A.J. Katan, Natascha Roest, Ute Kaiser, C. Dekker, Peter J. Peters, H.W. Zandbergen Files PDF s41598_019_43687_5.pdf 2.95 MB Close viewer /islandora/object/uuid:1b18711b-c3e0-430d-9701-05008b184588/datastream/OBJ/view