Statistical validation of megavariate effects in ASCA
article
Background: Innovative extensions of (M) ANOVA gain common ground for the analysis of designed metabolomics experiments. ASCA is such a multivariate analysis method; it has successfully estimated effects in megavariate metabolomics data from biological experiments. However, rigorous statistical validation of megavariate effects is still problematic because megavariate extensions of the classical F-test do not exist. Methods: A permutation approach is used to validate megavariate effects observed with ASCA. By permuting the class labels of the underlying experimental design, a distribution of no-effect is calculated. If the observed effect is clearly different from this distribution the effect is deemed significant. Results: The permutation approach is studied using simulated data which gave successful results. It was then used on real-life metabolomics data set dealing with bromobenzene-dosed rats. In this metabolomics experiment the dosage and time-interaction effect were validated, both effects are significant. Histological screening of the treated rats' liver agrees with this finding. Conclusion: The suggested procedure gives approximate p-values for testing effects underlying metabolomics data sets. Therefore, performing model validation is possible using the proposed procedure. © 2007 Vis et al; licensee BioMed Central Ltd.
Topics
Analytical researchbromobenzenearticlecontrolled studymathematical analysismathematical variablemetabolomicsmethodologymultivariate analysis of variancenonhumanprincipal component analysisprotein functionratstatistical analysisstatistical modelvalidation processanimaldose responsedrug effectgeneticsgenomicslivermetabolismmultivariate analysistimevalidation studyRattusAnimalsBromobenzenesData Interpretation, StatisticalDose-Response Relationship, DrugGenomicsLiverMetabolic Networks and PathwaysModels, StatisticalMultivariate AnalysisRatsTime Factors
TNO Identifier
240152
ISSN
14712105
Source
BMC Bioinformatics, 8
Article nr.
No.: 322
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