Print Email Facebook Twitter Biocatalytic C=C Bond Reduction through Carbon Nanodot-Sensitized Regeneration of NADH Analogues Title Biocatalytic C=C Bond Reduction through Carbon Nanodot-Sensitized Regeneration of NADH Analogues Author Kim, Jinhyun (Korea Advanced Institute of Science and Technology) Lee, S.H. (Korea Advanced Institute of Science and Technology) Tieves, F. (TU Delft BT/Biocatalysis) Choi, Da Som (Korea Advanced Institute of Science and Technology) Hollmann, F. (TU Delft BT/Biocatalysis) Paul, C.E. (Wageningen University & Research) Park, Chan Beum (Korea Advanced Institute of Science and Technology) Date 2018 Abstract Light-driven activation of redox enzymes is an emerging route for sustainable chemical synthesis. Among redox enzymes, the family of Old Yellow Enzyme (OYE) dependent on the nicotinamide adenine dinucleotide cofactor (NADH) catalyzes the stereoselective reduction of α,β-unsaturated hydrocarbons. Here, we report OYE-catalyzed asymmetric hydrogenation through light-driven regeneration of NADH and its analogues (mNADHs) by N-doped carbon nanodots (N-CDs), a zero-dimensional photocatalyst. Our spectroscopic and photoelectrochemical analyses verified the transfer of photo-induced electrons from N-CDs to an organometallic electron mediator (M) for highly regioselective regeneration of cofactors. Light triggered the reduction of NAD+ and mNAD+s with the cooperation of N-CDs and M, and the reduction behaviors of cofactors were dependent on their own reduction peak potentials. The regenerated cofactors subsequently delivered hydrides to OYE for stereoselective conversions of a broad range of substrates with excellent biocatalytic efficiencies. Subject alkene hydrogenationasymmetric catalysiscarbon nanodotNADH analoguesphotobiocatalysis To reference this document use: http://resolver.tudelft.nl/uuid:240f014a-a6f2-4225-866e-79582f481bca DOI https://doi.org/10.1002/anie.201804409 Embargo date 2019-01-30 ISSN 1433-7851 Source Angewandte Chemie (International Edition), 57 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2018 Jinhyun Kim, S.H. Lee, F. Tieves, Da Som Choi, F. Hollmann, C.E. Paul, Chan Beum Park Files PDF Kim_et_al_2018_Angewandte ... dition.pdf 1.69 MB Close viewer /islandora/object/uuid:240f014a-a6f2-4225-866e-79582f481bca/datastream/OBJ/view