Sustained release of BMP-2 in bioprinted alginate for osteogenicity in mice and rats

Sustained release of BMP-2 in bioprinted alginate for osteogenicity in mice and rats

Poldervaart, M.T.
Wang, H.
Van der Stok, J.
Weinans, H.H.
Leeuwenburgh, S.C.G.
Cumhur Öner, F.
Dhert, W.J.A.
Alblas, J.

Mechanical, Maritime and Materials Engineering

Biomechanical Engineering

2013-08-19

The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the sustained release of bone morphogenetic protein 2 (BMP-2) influences osteogenicity of tissue engineered bioprinted constructs. BMP-2 loaded on gelatin microparticles (GMPs) was used as a sustained release system, which was dispersed in hydrogel-based constructs and compared to direct inclusion of BMP-2 in alginate or control GMPs. The constructs were supplemented with goat multipotent stromal cells (gMSCs) and biphasic calcium phosphate to study osteogenic differentiation and bone formation respectively. BMP-2 release kinetics and bioactivity showed continuous release for three weeks coinciding with osteogenicity. Osteogenic differentiation and bone formation of bioprinted GMP containing constructs were investigated after subcutaneous implantation in mice or rats. BMP-2 significantly increased bone formation, which was not influenced by the release timing. We showed that 3D printing of controlled release particles is feasible and that the released BMP-2 directs osteogenic differentiation in vitro and in vivo.

http://resolver.tudelft.nl/uuid:266266e8-a237-431f-8cb3-54643dba3d5d

https://doi.org/10.1371/journal.pone.0072610

Public Library of Science PLOS

1932-6203

PloS ONE, 8 (8), 2013

Institutional Repository

journal article

(c) 2013 Poldervaart et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.