Fatigue is one of the common failure mechanisms occurring in asphalt pavements. To better understand the underlying principles of these fatigue mechanisms, a procedure is developed to construct fatigue curves based on tests in uni-axial tension on asphalt mortar, leading to defining fatigue life using a variety of methods. The uni-axial tension loading mode is chosen to increase the insight of material behavior. To examine discontinuous fatigue mechanisms, tests are performed to assess the healing behavior of asphalt mortar. Fatigue in asphalt mortar has not yet been investigated to a comprehensive extent so far. In mortar, it is expected that the behavior of the binder component is more pronounced when compared to asphalt mixtures. Mortar is a mixture of bitumen, filler, and fine sand with the ratio of 37.5 : 34.1 : 28.4. The bitumen used has pen grade 70/100, the used filler is Wigro 60K, and the sand is Norwegian sand sieved between 0.063 mm and 0.5 mm. Initially, a test procedure is formulated to determine a fatigue curve of mortar columns tested in uniaxial tension. Cylindrical specimen with a height of 63 mm and a diameter of 16.5 mm are tested in a pneumatic 5 kN UTM (Universal Testing Machine). Both the frequency and temperature are fixed at respectively 10 Hz and 5ºC for all these tests. The fatigue tests are performed in tension only, since this results in a relatively simple stress state in the cross-section. The tests are conducted using unaged specimens and four aged mortar specimens with varying ageing degrees. The ageing method used is temperature aged for 1, 2, 6, and 9 days in an oven at 85ºC. Every fatigue test is executed six times to account for relatively large variations of fatigue tests. After determining the fatigue curves, four different methods are applied to the fatigue test results in order to define the fatigue life of asphalt mortar. The classical method in pavement engineering is applied first, which defines the fatigue life when reaching 50% of the initial stiffness. Secondly, the fatigue life is defined at the transition point between Phase II and Phase III. This point is the maximum of the stiffness multiplied by the number of load cycles plotted against the number of load cycles. This is a mathematical approach instead of looking into the material behavior of asphalt mortar. The third method is based on the dissipated energy of each load cycle during a fatigue test using the formula Van Dijk postulated, an analytical approach in which the area of the stress-strain curve is defined as the dissipated energy. The fourth and final method is using the monotonic tensile test results as a failure envelope to the fatigue results, normally applied to linear elastic materials and is not commonly applied in pavement engineering. The transition point approach shows results close to the actual fracture of the specimens. The definition of the fatigue life based on the dissipated energy results in a relatively low number of load cycles until fatigue failure occurs, but this is thought to be due to the available data and the applied data analysis. Overall, it can be concluded that using the transition point is the best method to define the fatigue life based on the experimental data found. The 2 days aged mortar specimens show the highest number of load cycles, which is not in line with the expectations. The fatigue life was expected to decrease as the ageing period increased, which can be seen in the 2, 6, and 9 days aged mortar specimens. It is believed that performing fatigue tests on unaged mortar is not useful, since the material is too soft. To assess healing behavior, discontinuous tests are performed using the same test set-up and parameters as in the cyclic fatigue tests. In the rest periods between the load cycles, the asphalt mortar has an opportunity to heal. Various loading patterns are applied to see their effect on the fatigue life and to determine the healing factor. The healing factor was found to be relatively low, but for a test temperature of 5ºC it is thought to be a realistic value. It is found that the duration of the rest period does not increase the healing factor as expected.