Print Email Facebook Twitter Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis Title Interaction of storage carbohydrates and other cyclic fluxes with central metabolism: A quantitative approach by non-stationary 13C metabolic flux analysis Author Suarez Mendez, C.A. Hanemaaijer, M. Ten Pierick, A. Wolters, J.C. Heijnen, J.J. Wahl, S.A. Faculty Applied Sciences Department BT/Biotechnology Date 2016-01-22 Abstract 13C labeling experiments in aerobic glucose limited cultures of Saccharomyces cerevisiae at four different growth rates (0.054; 0.101, 0.207, 0.307 h?1) are used for calculating fluxes that include intracellular cycles (e.g., storage carbohydrate cycles, exchange fluxes with amino acids), which are rearranged depending on the growth rate. At low growth rates the impact of the storage carbohydrate recycle is relatively more significant than at high growth rates due to a higher concentration of these materials in the cell (up to 560-fold) and higher fluxes relative to the glucose uptake rate (up to 16%). Experimental observations suggest that glucose can be exported to the extracellular space, and that its source is related to storage carbohydrates, most likely via the export and subsequent extracellular breakdown of trehalose. This hypothesis is strongly supported by 13C-labeling experimental data, measured extracellular trehalose, and the corresponding flux estimations. Subject non-stationary 13C labelingflux estimationtrehaloseglycogenamino acids To reference this document use: http://resolver.tudelft.nl/uuid:2c5a4e2d-18f1-4739-a590-02038613964d Publisher Elsevier ISSN 2214-0301 Source https://doi.org/10.1016/j.meteno.2016.01.001 Source Metabolic Engineering Communications, 3, 2016 Part of collection Institutional Repository Document type journal article Rights (c) 2016 International Metabolic Engineering SocietyUnder a Creative Commons license Files PDF 329134.pdf 879.55 KB Close viewer /islandora/object/uuid:2c5a4e2d-18f1-4739-a590-02038613964d/datastream/OBJ/view