Effect of temperature and fixation on the optical properties of atherosclerotic tissue: A validation study of an ex-vivo whole heart cadaveric model

Effect of temperature and fixation on the optical properties of atherosclerotic tissue: A validation study of an ex-vivo whole heart cadaveric model

Gnanadesigan, M.
Van Soest, G.
White, S.
Scoltock, S.
Ughi, G.J.
Baumbach, A.
Van der Steen, A.F.W.
Regar, E.
Johnson, T.W.

Applied Sciences

ImPhys/Imaging Physics

2014-03-03

Atherosclerotic plaque composition can be imaged using the optical attenuation coefficient derived from intravascular optical coherence tomography (OCT) data. The relation between optical properties and tissue type has been established on autopsy tissues. In this study, we validate an ex-vivo model for the effect of temperature and tissue fixation on optical parameters. We studied the optical attenuation of human coronary arteries at three temperatures, before and after formalin fixation. We developed an en-face longitudinal display of attenuation data of the OCT pullbacks. Using the unfixed, body-temperature condition image as a standard, and after extensive registration with other condition images, we quantify the differences in optical attenuation and the backscattered intensity. The results suggest that tissue fixation and temperature do not introduce systematic errors in studies of arterial optical properties.

image analysis
image reconstruction techniques
medical and biological imaging
optical coherence tomography

http://resolver.tudelft.nl/uuid:2e3cbde5-a412-45f4-a5f4-df41f142159f

https://doi.org/10.1364/BOE.5.001038

Optical Society of America

2156-7085

http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-5-4-1038

Biomedical Optics Express, 5 (4), 2014

Institutional Repository

journal article

(c) 2014 Optical Society of America