Print Email Facebook Twitter Combined Confocal Microscope and Brandaris 128 Ultra-High-Speed Camera Title Combined Confocal Microscope and Brandaris 128 Ultra-High-Speed Camera Author Beekers, D.I. (Erasmus MC) Lattwein, Kirby R. (Erasmus MC) Kouijzer, Joop J.P. (Erasmus MC) Langeveld, Simone A.G. (Erasmus MC) Vegter, M. (Erasmus MC) Beurskens, Robert (Erasmus MC) Mastik, F. (Erasmus MC) Verduyn Lunel, Rogier (Nikon, Amsterdam) van der Steen, A.F.W. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC) de Jong, N. (TU Delft ImPhys/Acoustical Wavefield Imaging; Erasmus MC) Date 2019 Abstract Controlling microbubble-mediated drug delivery requires the underlying biological and physical mechanisms to be unraveled. To image both microbubble oscillation upon ultrasound insonification and the resulting cellular response, we developed an optical imaging system that can achieve the necessary nanosecond temporal and nanometer spatial resolutions. We coupled the Brandaris 128 ultra-high-speed camera (up to 25 million frames per second) to a custom-built Nikon A1R+ confocal microscope. The unique capabilities of this combined system are demonstrated with three experiments showing microbubble oscillation leading to either endothelial drug delivery, bacterial biofilm disruption, or structural changes in the microbubble coating. In conclusion, using this state-of-the-art optical imaging system, microbubble-mediated drug delivery can be studied with high temporal resolution to resolve microbubble oscillation and high spatial resolution and detector sensitivity to discern cellular response. Combining these two imaging technologies will substantially advance our knowledge on microbubble behavior and its role in drug delivery. Subject BacteriaConfocal microscopyDrug deliveryFluorescence microscopyHigh-speed imagingLipid coatingMicrobubbleSonoporationUltrasoundUltrasound contrast agents To reference this document use: http://resolver.tudelft.nl/uuid:361f73a0-9c25-4e68-9671-9eed129df834 DOI https://doi.org/10.1016/j.ultrasmedbio.2019.06.004 ISSN 0301-5629 Source Ultrasound in Medicine & Biology, 45 (9), 2575-2582 Part of collection Institutional Repository Document type journal article Rights © 2019 D.I. Beekers, Kirby R. Lattwein, Joop J.P. Kouijzer, Simone A.G. Langeveld, M. Vegter, Robert Beurskens, F. Mastik, Rogier Verduyn Lunel, A.F.W. van der Steen, N. de Jong Files PDF 1_s2.0_S0301562919302698_main.pdf 2.19 MB Close viewer /islandora/object/uuid:361f73a0-9c25-4e68-9671-9eed129df834/datastream/OBJ/view