Print Email Facebook Twitter Integration of Gas Enhanced Oil Recovery in Multiphase Fermentations for the Microbial Production of Fuels and Chemicals Title Integration of Gas Enhanced Oil Recovery in Multiphase Fermentations for the Microbial Production of Fuels and Chemicals Author Pedraza de la Cuesta, S. (TU Delft BT/Bioprocess Engineering) Keijzers, L. (TU Delft Applied Sciences) van der Wielen, L.A.M. (TU Delft BT/Bioprocess Engineering; Bernal Institute) Cuellar Soares, M.C. (TU Delft BT/Bioprocess Engineering) Faculty Applied Sciences Date 2018 Abstract In multiphase fermentations where the product forms a second liquid phase or where solvents are added for product extraction, turbulent conditions disperse the oil phase as droplets. Surface-active components (SACs) present in the fermentation broth can stabilize the product droplets thus forming an emulsion. Breaking this emulsion increases process complexity and consequently the production cost. In previous works, it has been proposed to promote demulsification of oil/supernatant emulsions in an off-line batch bubble column operating at low gas flow rate. The aim of this study is to test the performance of this recovery method integrated to a fermentation, allowing for continuous removal of the oil phase. A 500 mL bubble column is successfully integrated with a 2 L reactor during 24 h without affecting cell growth or cell viability. However, higher levels of surfactants and emulsion stability are measured in the integrated system compared to a base case, reducing its capacity for oil recovery. This is related to release of SACs due to cellular stress when circulating through the recovery column. Therefore, it is concluded that the gas bubble-induced oil recovery method allows for oil separation and cell recycling without compromising fermentation performance; however, tuning of the column parameters considering increased levels of SACs due to cellular stress is required for improving oil recovery. Subject emulsionfermentationintegrationmicrobial biofuelsproduct recovery To reference this document use: http://resolver.tudelft.nl/uuid:3717db39-120d-40d5-b034-cb0a7f5dafc8 DOI https://doi.org/10.1002/biot.201700478 ISSN 1860-6768 Source Biotechnology Journal, 13 (4) Part of collection Institutional Repository Document type journal article Rights © 2018 S. Pedraza de la Cuesta, L. Keijzers, L.A.M. van der Wielen, M.C. Cuellar Soares Files PDF Cuesta_et_al_2018_Biotech ... ournal.pdf 1.34 MB Close viewer /islandora/object/uuid:3717db39-120d-40d5-b034-cb0a7f5dafc8/datastream/OBJ/view