Print Email Facebook Twitter Unfocused shockwaves for osteoinduction in bone substitutes in rat cortical bone defects Title Unfocused shockwaves for osteoinduction in bone substitutes in rat cortical bone defects Author Koolen, Marianne K.E. (University Medical Center Utrecht) Pouran, B. (TU Delft Biomaterials & Tissue Biomechanics; University Medical Center Utrecht) Oner, Fetullah C. (University Medical Center Utrecht) Zadpoor, A.A. (TU Delft Biomaterials & Tissue Biomechanics) van der Jagt, Olav P. (Elisabeth-TweeSteden Hospital, Tilburg) Weinans, Harrie (TU Delft Biomaterials & Tissue Biomechanics; University Medical Center Utrecht) Date 2018 Abstract Bone substitutes are frequently used in clinical practice but often exhibit limited osteoinductivity. We hypothesized that unfocused shockwaves enhance the osteoinductivity of bone substitutes and improve osteointegration and angiogenesis. Three different bone substitutes, namely porous tricalcium phosphate, porous hydroxyapatite and porous titanium alloy, were implanted in a critical size (i.e. 6-mm) femoral defect in rats. The femora were treated twice with 1500 shockwaves at 2 and 4 weeks after surgery and compared with non-treated controls. The net volume of de novo bone in the defect was measured by microCT-scanning during 11-weeks follow-up. Bone ingrowth and angiogenesis in the bone substitutes was examined at 5 and 11 weeks using histology. It was shown that hydroxyapatite and titanium both had an increase of bone ingrowth with more bone in the shockwave group compared to the control group, whereas resorption was seen in tricalcium phosphate bone substitutes over time and this was insensitive to shockwave treatment. In conclusion, hydroxyapatite and titanium bone substitutes favour from shockwave treatment, whereas tricalcium phosphate does not. This study shows that osteoinduction and osteointegration of bone substitutes can be influenced with unfocused shockwave therapy, but among other factors depend on the type of bone substitute, likely reflecting its mechanical and biological properties. To reference this document use: http://resolver.tudelft.nl/uuid:3fe1f9b9-d7ba-419d-97dc-d2f60cd44928 DOI https://doi.org/10.1371/journal.pone.0200020 ISSN 1932-6203 Source PLoS ONE, 13 (7) Part of collection Institutional Repository Document type journal article Rights © 2018 Marianne K.E. Koolen, B. Pouran, Fetullah C. Oner, A.A. Zadpoor, Olav P. van der Jagt, Harrie Weinans Files PDF journal.pone.0200020.pdf 24.49 MB Close viewer /islandora/object/uuid:3fe1f9b9-d7ba-419d-97dc-d2f60cd44928/datastream/OBJ/view