Print Email Facebook Twitter Light-Harvesting Dye-Alginate Hydrogel for Solar-Driven, Sustainable Biocatalysis of Asymmetric Hydrogenation Title Light-Harvesting Dye-Alginate Hydrogel for Solar-Driven, Sustainable Biocatalysis of Asymmetric Hydrogenation Author Yoon, Jaeho (Korea Advanced Institute of Science and Technology) Lee, S.H. (Korea Advanced Institute of Science and Technology) Tieves, F. (TU Delft BT/Biocatalysis) Rauch, M.C.R. (TU Delft BT/Biocatalysis) Hollmann, F. (TU Delft BT/Biocatalysis) Park, Chan Beum (Korea Advanced Institute of Science and Technology) Date 2019 Abstract We report visible light-driven, asymmetric hydrogenation of C=C bonds using an ene-reductase from Thermus scotoductus SA-01 (TsOYE) and a light-harvesting dye (rose bengal, RB) co-immobilized in an alginate hydrogel. Highly efficient encapsulation of RB in alginate hydrogel was achieved using the intrinsic affinity between TsOYE and RB, which allowed for the construction of robust RB-TsOYE-loaded alginate capsules. In the absence of NADH, the photobiocatalytic system facilitated asymmetric reduction of 2-methylcyclohexenone to an enantiopure (R)-2-methylcyclohexanone (ee > 99%; max. conversion, 70.4%; turnover frequency, 1.54 min -1 turnover number, 300.2) under illumination. A series of stability tests revealed a significant enhancement of TsOYE's robustness in alginate hydrogel against heat and chemical denaturants. This study provides insight into a greener and sustainable approach of cofactor-free OYE catalysis for producing value-added chemicals using light energy. Subject Alginate hydrogelAsymmetric hydrogenationEne-reductaseNADH-free bioprocessPhotobiocatalysis To reference this document use: http://resolver.tudelft.nl/uuid:41496265-e2e1-424d-b02a-9bc5f0ab04ff DOI https://doi.org/10.1021/acssuschemeng.9b01075 Embargo date 2019-09-03 ISSN 2168-0485 Source ACS Sustainable Chemistry and Engineering, 7 (6), 5632-5637 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2019 Jaeho Yoon, S.H. Lee, F. Tieves, M.C.R. Rauch, F. Hollmann, Chan Beum Park Files PDF acssuschemeng.9b01075.pdf 2.17 MB Close viewer /islandora/object/uuid:41496265-e2e1-424d-b02a-9bc5f0ab04ff/datastream/OBJ/view