Print Email Facebook Twitter Developing a tool for the validation of quantitative DCE-MRI Title Developing a tool for the validation of quantitative DCE-MRI Author Bol, K. Haeck, J.C. Alic, L. Bernsen, M. De Jong, M. Niessen, W.J. Veenland, J.F. Faculty Applied Sciences Date 2011-03-11 Abstract Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) is becoming an indispensable tool to noninvasively study tumor characteristics. However, many different DCE-analysis methods are currently being used. To compare and validate different methods, histology is the gold standard. For this purpose, exact co-localization between histology and MRI images is a prerequisite. In this study a methodology is developed to validate DCE-data with histology with an emphasis on correct registration of DCE-MRI and histological data. A pancreatic tumor was grown in a rat model. The tumor was dissected after MR imaging, embedded in paraffin, and cut into thin slices. These slices were stained with haematoxylin and eosin, digitized and stacked in a 3D volume. Next, the 3D histology was registered to exvivo SWI-weighted MR images, which in turn were registered to in-vivo SWI and DCE images to achieve correct colocalization. Semi-quantitative and quantitative parameters were calculated. Preliminary results suggest that both pharmacokinetic and heuristic DCE-parameters can discriminate between vital and non-vital tumor regions. The developed method offers the basis for an accurate spatial correlation between DCE-MRI derived parametric maps and histology, and facilitates the evaluation of different DCE-MRI analysis methods. Subject DCE-MRI, histology, registration, DCE-analysis, quantitative, semi-quantitative, pharmacokinetic modeling,two-compartment model To reference this document use: http://resolver.tudelft.nl/uuid:4230b0e2-29eb-45f1-ba96-7ab99c7fbc07 DOI https://doi.org/10.1117/12.877834 Publisher SPIE ISSN 0277-786X Source https://doi.org/10.1117/12.877834 Source Proceedings of SPIE, 2011 vol. 7965 Part of collection Institutional Repository Document type conference paper Rights (c)2011 The Authors Files PDF 2011-7965.Bol.pdf 1.45 MB Close viewer /islandora/object/uuid:4230b0e2-29eb-45f1-ba96-7ab99c7fbc07/datastream/OBJ/view