Print Email Facebook Twitter Paving the way to single-molecule protein sequencing Title Paving the way to single-molecule protein sequencing Author Restrepo Perez, L. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) Joo, C. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) Dekker, C. (TU Delft BN/Cees Dekker Lab; Kavli institute of nanoscience Delft) Date 2018 Abstract Proteins are major building blocks of life. The protein content of a cell and an organism provides key information for the understanding of biological processes and disease. Despite the importance of protein analysis, only a handful of techniques are available to determine protein sequences, and these methods face limitations, for example, requiring a sizable amount of sample. Single-molecule techniques would revolutionize proteomics research, providing ultimate sensitivity for the detection of low-abundance proteins and the realization of single-cell proteomics. In recent years, novel single-molecule protein sequencing schemes that use fluorescence, tunnelling currents and nanopores have been proposed. Here, we present a review of these approaches, together with the first experimental efforts towards their realization. We discuss their advantages and drawbacks, and present our perspective on the development of single-molecule protein sequencing techniques. To reference this document use: http://resolver.tudelft.nl/uuid:4333d3be-bfdd-4770-92de-fafa2dd1deca DOI https://doi.org/10.1038/s41565-018-0236-6 Embargo date 2019-03-06 ISSN 1748-3387 Source Nature Nanotechnology, 13 (9), 786-796 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type review Rights © 2018 L. Restrepo Perez, C. Joo, C. Dekker Files PDF ReviewSMPS_v20.pdf 2.79 MB Close viewer /islandora/object/uuid:4333d3be-bfdd-4770-92de-fafa2dd1deca/datastream/OBJ/view