Print Email Facebook Twitter A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion in S. Cerevisiae Title A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion in S. Cerevisiae Author Sun, Su Ming (Leiden University Medical Center) Batté, Amandine (Leiden University Medical Center) Elmer, Mireille (TU Delft Quantum & Computer Engineering; Leiden University Medical Center) van der Horst, Sophie C. (Leiden University Medical Center) van Welsem, Tibor (Netherlands Cancer Institute) Bean, Gordon (University of California) Ideker, Trey (University of California; Moores UCSD Cancer Center) van Leeuwen, Fred (Netherlands Cancer Institute) van Attikum, Haico (Leiden University Medical Center) Department Quantum & Computer Engineering Date 2020 Abstract Eukaryotic chromosomes are replicated in interphase and the two newly duplicated sister chromatids are held together by the cohesin complex and several cohesin auxiliary factors. Sister chromatid cohesion is essential for accurate chromosome segregation during mitosis, yet has also been implicated in other processes, including DNA damage repair, transcription and DNA replication. To assess how cohesin and associated factors functionally interconnect and coordinate with other cellular processes, we systematically mapped the genetic interactions of 17 cohesin genes centered on quantitative growth measurements of >52,000 gene pairs in the budding yeast Saccharomyces cerevisiae. Integration of synthetic genetic interactions unveiled a cohesin functional map that constitutes 373 genetic interactions, revealing novel functional connections with post-replication repair, microtubule organization and protein folding. Accordingly, we show that the microtubule-associated protein Irc15 and the prefoldin complex members Gim3, Gim4 and Yke2 are new factors involved in sister chromatid cohesion. Our genetic interaction map thus provides a unique resource for further identification and functional interrogation of cohesin proteins. Since mutations in cohesin proteins have been associated with cohesinopathies and cancer, it may also help in identifying cohesin interactions relevant in disease etiology. Subject CohesinCohesinopathyGenetic interaction mappingIrc15PrefoldinSister chromatid cohesion To reference this document use: http://resolver.tudelft.nl/uuid:437dbafd-1869-442d-85d7-04f1124f5e14 DOI https://doi.org/10.1242/jcs.237628 ISSN 0021-9533 Source Journal of Cell Science, 133 (10) Part of collection Institutional Repository Document type journal article Rights © 2020 Su Ming Sun, Amandine Batté, Mireille Elmer, Sophie C. van der Horst, Tibor van Welsem, Gordon Bean, Trey Ideker, Fred van Leeuwen, Haico van Attikum Files PDF jcs237628.full.pdf 6.61 MB Close viewer /islandora/object/uuid:437dbafd-1869-442d-85d7-04f1124f5e14/datastream/OBJ/view