Print Email Facebook Twitter Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An In Vitro and In Vivo Stability Study Title Radioactive Holmium Acetylacetonate Microspheres for Interstitial Microbrachytherapy: An In Vitro and In Vivo Stability Study Author Bult, W. De Leeuw, H. Steinebach, O.M. Van der Bom, M.J. Wolterbeek, H.T. Heeren, R.M.A. Bakker, C.J.G. Van het Schip, A.D. Hennink, W.E. Nijsen, F.W. Faculty Applied Sciences Department Radiation, Radionuclides and Reactors Date 2011-11-09 Abstract Purpose The clinical application of holmium acetylacetonate microspheres (HoAcAcMS) for the intratumoral radionuclide treatment of solid malignancies requires a thorough understanding of their stability. Therefore, an in vitro and an in vivo stability study with HoAcAcMS was conducted. Methods HoAcAcMS, before and after neutron irradiation, were incubated in a phosphate buffer at 37°C for 6 months. The in vitro release of holmium in this buffer after 6 months was 0.5%. Elemental analysis, scanning electron microscopy, infrared spectroscopy and time of flight secondary ion mass spectrometry were performed on the HoAcAcMS. Results After 4 days in buffer the acetylacetonate ligands were replaced by phosphate, without altering the particle size and surface morphology. HoAcAcMS before and after neutron irradiation were administered intratumorally in VX2 tumor-bearing rabbits. No holmium was detected in the faeces, urine, femur and blood. Histological examination of the tumor revealed clusters of intact microspheres amidst necrotic tissue after 30 days. Conclusion HoAcAcMS are stable both in vitro and in vivo and are suitable for intratumoral radionuclide treatment. Subject brachytherapyholmiumin vivomicrospheresVX2 carcinoma To reference this document use: http://resolver.tudelft.nl/uuid:5228f2ad-7969-4e5e-97a8-bbdbbf49d5bd DOI https://doi.org/10.1007/s11095-011-0610-7 Publisher Springer ISSN 0724-8741 Source http://www.springerlink.com/content/66376578k525tj46/ Source Pharmaceutical Research, 29 (3), 2012 Part of collection Institutional Repository Document type journal article Rights (c) 2011 The Author(s). This article is published with open access at Springerlink.com Files PDF bult.pdf 576.38 KB Close viewer /islandora/object/uuid:5228f2ad-7969-4e5e-97a8-bbdbbf49d5bd/datastream/OBJ/view