LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone

article
2001
Visser, J.

Graffelman, W.

Blauw, B.

Haspels, I.

Lentjes, E.

Kloet, de R.

Nagelkerken, L.
Several causes have been held responsible for the chronic fatigue syndrome (CFS), including an altered hypothalamus-pituitary-adrenal gland (HPA)-axis activity, viral infections and a reduced Th1 activity. Therefore, it was investigated whether the regulation of IL-10 is different in CFS. LPS-induced cytokine secretion in whole blood cultures showed a significant increase in IL-10 and a trend towards a decrease in IL-12 as compared with healthy controls. In patients and controls, IL-12 secretion was equally sensitive to suppression by dexamethasone, whereas IL-10 secretion appeared more sensitive in CFS-patients. In controls, IL-10 and IL-12 secretion were inversely correlated with free serum cortisol (r=-0.492, p<0.02 and r=-0.434, p<0.05, respectively). In CFS, such an inverse correlation was found for IL-12 (r=-0.611, p<0.02) but not for IL-10 (r=-0.341, ns). These data are suggestive for a disturbed glucocorticoid regulation of IL-10 in CFS. © 2001 Elsevier Science B.V. All rights reserved. Chemicals/CAS: Dexamethasone, 50-02-2; Glucocorticoids; Hydrocortisone, 50-23-7; Interleukin-10, 130068-27-8; Interleukin-12, 187348-17-0; Lipopolysaccharides; RNA, Messenger; Tumor Necrosis Factor-alpha
Topics
Chronic fatigue syndromeCortisolDexamethasoneGlucocorticoidsIL-10IL-12TNF-αViral IL-10Bacterium lipopolysaccharidBlood cultureChemosensitivityChronic fatigue syndromeControlled studyCorrelation functionCytokine productionCytokine releaseDrug blood levelDrug effectFemaleHypothalamus hypophysis systemImmunoregulationMajor clinical studyMaleAdolescentAdultCells, CulturedDexamethasoneFatigue Syndrome, ChronicGene ExpressionGlucocorticoidsHumansHydrocortisoneInterleukin-10Interleukin-12LeukocytesLipopolysaccharidesMiddle AgedRNA, MessengerTumor Necrosis Factor-alpha
TNO Identifier
236239
Repository link
https://resolver.tno.nl/uuid:5470142c-fd6a-4fe6-a2e3-dc9f7722189b
ISSN
01655728
Source
Journal of Neuroimmunology, 119(2), pp. 343-349.
Pages
343-349
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