Print Email Facebook Twitter Single-cell analysis of the Dps response to oxidative stress Title Single-cell analysis of the Dps response to oxidative stress Author De Martino, M. (TU Delft BN/Anne Meyer Lab; Kavli institute of nanoscience Delft) Ershov, D. (Systems Biophysics) van den Berg, P.J. (TU Delft Applied Sciences; Kavli institute of nanoscience Delft) Tans, S.J. (AMOLF) Meyer, A.S. (TU Delft BN/Anne Meyer Lab; Kavli institute of nanoscience Delft) Faculty Applied Sciences Date 2016-06-01 Abstract Microorganisms have developed an elaborate spectrum of mechanisms to respond and adapt to environmental stress conditions. Among these is the expression of dps, coding for the DNA-binding protein from starved cells. Dps becomes the dominant nucleoid- organizing protein in stationary-phase Escherichia coli cells and is required for robust survival under stress conditions, including carbon or nitrogen starvation, oxidative stress, metal exposure, and irradiation. To study the complex regulation of Dps in E. coli, we utilized time-lapse fluorescence microscopy imaging to examine the kinetics, input encoding, and variability of the Dps response in single cells. In the presence of an oxidative stressor, we observed a single pulse of activation of Dps production. Increased concentrations of H2O2 led to increased intensity and duration of the pulse. While lower concentrations of H2O2 robustly activated the Dps response with little effect on the growth rate, higher concentrations of H2O2 resulted in dramatically lower and highly varied growth rates. A comparison of cells exposed to the same concentration of H2O2 revealed that increased levels of Dps expression did not confer a growth advantage, indicating that recovery from stress may rely primarily upon variation in the amount of damage caused to individual cells. To reference this document use: http://resolver.tudelft.nl/uuid:560b2120-aae9-4b44-b6d5-3fbf1108d74f DOI https://doi.org/10.1128/JB.00239-16 ISSN 0021-9193 Source Journal of Bacteriology, 198 (11), 1662-1674 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2016 M. De Martino, D. Ershov, P.J. van den Berg, S.J. Tans, A.S. Meyer Files PDF DeMartinoMeyerJBac2016Tex ... igures.pdf 2.58 MB Close viewer /islandora/object/uuid:560b2120-aae9-4b44-b6d5-3fbf1108d74f/datastream/OBJ/view