Print Email Facebook Twitter Mechanical force induces mitochondrial fission Title Mechanical force induces mitochondrial fission Author Helle, Sebastian Carsten Johannes (ETH Zürich) Feng, Q. (ETH Zürich) Aebersold, Mathias J. (ETH Zürich) Hirt, Luca (ETH Zürich) Grüter, Raphael R. (ETH Zürich) Vahid Belarghou, A. (TU Delft BN/Timon Idema Lab; Kavli institute of nanoscience Delft) Sirianni, Andrea (Imperial College London) Mostowy, Serge (Imperial College London) Snedeker, Jess G. (University of Zürich; ETH Zürich) Šarić, Anđela (University College London (UCL)) Idema, T. (TU Delft BN/Timon Idema Lab; Kavli institute of nanoscience Delft) Zambelli, Tomaso (ETH Zürich) Kornmann, Benoît (ETH Zürich) Date 2017-11-09 Abstract Eukaryotic cells are densely packed with macromolecular complexes and intertwining organelles, continually transported and reshaped. Intriguingly, organelles avoid clashing and entangling with each other in such limited space. Mitochondria form extensive networks constantly remodeled by fission and fusion. Here, we show that mitochondrial fission is triggered by mechanical forces. Mechano-stimulation of mitochondria - via encounter with motile intracellular pathogens, via external pressure applied by an atomic force microscope, or via cell migration across uneven microsurfaces - results in the recruitment of the mitochondrial fission machinery, and subsequent division. We propose that MFF, owing to affinity for narrow mitochondria, acts as a membrane-bound force sensor to recruit the fission machinery to mechanically strained sites. Thus, mitochondria adapt to the environment by sensing and responding to biomechanical cues. Our findings that mechanical triggers can be coupled to biochemical responses in membrane dynamics may explain how organelles orderly cohabit in the crowded cytoplasm. To reference this document use: http://resolver.tudelft.nl/uuid:568363df-1e8f-40f2-a1dd-31b187fc8b6a DOI https://doi.org/10.7554/eLife.30292 ISSN 2050-084X Source eLife, 6, 1-26 Part of collection Institutional Repository Document type journal article Rights © 2017 Sebastian Carsten Johannes Helle, Q. Feng, Mathias J. Aebersold, Luca Hirt, Raphael R. Grüter, A. Vahid Belarghou, Andrea Sirianni, Serge Mostowy, Jess G. Snedeker, Anđela Šarić, T. Idema, Tomaso Zambelli, Benoît Kornmann Files PDF elife_30292_v1.pdf 5.93 MB Close viewer /islandora/object/uuid:568363df-1e8f-40f2-a1dd-31b187fc8b6a/datastream/OBJ/view