Surface-cell interactions: Can PEO topography outsmart surface calcium?

Titanium surfaces modified by plasma electrolytic oxidation (PEO) have attractive physico-chemical characteristics that may favor osseointegration of bone implants. This study aimed at delineating the effects of PEO topography and surface calcium on the in vitro pre-osteoblasts response. Two different surfaces were generated by varying the oxidation time of Ti6Al7Nb alloy from 1 minute (PEO-3Ca) to 5 minutes (PEO-9Ca) in a calcium phosphate based electrolyte. These surfaces were compared to another surface without calcium (PEO-0Ca) yet with controlled topography produced by oxidation of Ti6Al7Nb alloy in a phosphorous based electrolyte. The in vitro response of pre-osteoblasts (SV-HFO) on these surfaces clearly indicated that surface calcium is not the main player in influencing cells behaviour as the growth, differentiation and mineralization of pre-osteoblasts were not affected on surface without calcium (PEO-0Ca). Furthermore, at initial stages the pre-osteoblasts response to surface without calcium was even enhanced than on the surface that had high calcium (PEO-9Ca). These findings show that surface calcium is not the key factor in promoting cells response and suggests that PEO topography can be a strong surface cue for modulating cells response.

Subject

Ti6Al7Nb
SV-HFO
growth
differentiation
mineralization

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http://resolver.tudelft.nl/uuid:5cf09033-4da3-420b-8ae3-ac3f3a02cd84

Embargo date

2017-08-21

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Student theses

Document type

master thesis

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Thesis_Delihta_Fernando.pdf

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