Print Email Facebook Twitter Ureohydrolases as dominant selectable markers in yeast Title Ureohydrolases as dominant selectable markers in yeast Author Daran, J.G. Pronk, J.T. Romagnoli, G. Faculty Applied Sciences Department BT/Biotechnology Date 2015-10-15 Abstract The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a microorganism having an altered genome. The invention further relates to a set of constructs, comprising a first construct comprising a recognition site for an endonuclease, a first region of homology with a target gene of a microorganism, and a first part of a nucleotide sequence encoding the selection marker, and a second construct comprising a second part of the nucleotide sequence encoding the selection marker, a second region of homology with the target gene of the microorganism, and a copy of the endonuclease recognition site. The invention further relates to methods for altering a target gene in a microorganism, to methods for producing a microorganism, and to microorganisms that are produced by the methods of the invention. To reference this document use: http://resolver.tudelft.nl/uuid:63c57205-336a-492f-8a8b-52e2650fa322 Publisher European Patent Office Source http://worldwide.espacenet.com/publicationDetails/biblio?DB=EPODOC&II=27&ND=3&adjacent=true&locale=en_EP&FT=D&date=20151015&CC=WO&NR=2015156675A1&KC=A1 Source WO2015156675 (A1) Part of collection Institutional Repository Document type patent Rights (c) 2015 The Author(s) Files PDF WO2015156675A1.pdf 2.9 MB Close viewer /islandora/object/uuid:63c57205-336a-492f-8a8b-52e2650fa322/datastream/OBJ/view