Sensorless Adaptive Optics in Confocal Microscopy

Sensorless Adaptive Optics in Confocal Microscopy

Gislason, Johann (TU Delft Mechanical, Maritime and Materials Engineering)

Delft University of Technology

Mechanical Engineering | Systems and Control

2019-03-20

Anisoplanatic corrections of wavefront aberrations in confocal microscopy is a difficult problem in Adaptive Optics (AO). Isoplanatic correction on the excitation- and fluorescence signals is commonly applied in traditional adaptive optics by using a Deformable Mirror (DM), but ideally, anisoplanatic corrections should be applied because anisoplanatic wavefront aberrations are common in reality. The problem of correcting the fluorescence anisoplanatically remains unsolved in AO, but recently a method was proposed that allows for anisoplanatic correction on the excitation signal. This task requires the solution of a complicated optimization problem, one that has so far been solved with a simple local search algorithm, and a better algorithm can be developed to further improve the method. In this work, a recently developed DONE algorithm is applied in the task. It is shown that the DONE algorithm performs better in the task than the original method.

http://resolver.tudelft.nl/uuid:6456d6bd-0879-4e47-bded-dee727ffa9ab

Student theses

master thesis

© 2019 Johann Gislason