Why Argonaute is needed to make microRNA target search fast and reliable

Title

Why Argonaute is needed to make microRNA target search fast and reliable

 Author

Klein, M. (TU Delft BN/Martin Depken Lab; Kavli institute of nanoscience Delft)
Chandradoss, S.D. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft)
Depken, S.M. (TU Delft BN/Martin Depken Lab; Kavli institute of nanoscience Delft) ORCID 0000-0001-9194-6739
Joo, C. (TU Delft BN/Chirlmin Joo Lab; Kavli institute of nanoscience Delft) ORCID 0000-0003-2803-0335

 Date

2017

 Abstract

MicroRNA (miRNA) interferes with the translation of cognate messenger RNA (mRNA) by finding, preferentially binding, and marking it for degradation. To facilitate the search process, Argonaute (Ago) proteins come together with miRNA, forming a dynamic search complex. In this review we use the language of free-energy landscapes to discuss recent single-molecule and high-resolution structural data in the light of theoretical work appropriated from the study of transcription-factor search. We suggest that experimentally observed internal states of the Ago-miRNA search complex may have the explicit biological function of speeding up search while maintaining specificity.


 Subject

Argonaute
Free-energy landscape
FRET
Lateral diffusion
MicroRNA target prediction
MiRNA
RNA silencing
Search stability paradox
Single molecule
Target Search
Theoretical model

 To reference this document use:

http://resolver.tudelft.nl/uuid:68993f5d-ae64-484b-8689-05d69f5cb7ea

 DOI

https://doi.org/10.1016/j.semcdb.2016.05.017

 ISSN

1084-9521

 Source

Seminars in Cell and Developmental Biology, 65, 20-28

 Part of collection

Institutional Repository

 Document type

journal article

 Rights

© 2017 M. Klein, S.D. Chandradoss, S.M. Depken, C. Joo
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