Print Email Facebook Twitter Imaging modes of atomic force microscopy for application in molecular and cell biology Title Imaging modes of atomic force microscopy for application in molecular and cell biology Author Dufrêne, Yves F. (Université Catholique de Louvain) Ando, Toshio (Kanazawa University) Garcia, Ricardo (Instituto de Ciencia de Materiales de Madrid (ICMM)) Alsteens, David (Université Catholique de Louvain) Martinez-Martin, David (ETH Zürich) Engel, A.H. (TU Delft BN/Andreas Engel Lab) Gerber, Christoph (University of Basel) Müller, Daniel J. (ETH Zürich) Date 2017-05-01 Abstract Atomic force microscopy (AFM) is a powerful, multifunctional imaging platform that allows biological samples, from single molecules to living cells, to be visualized and manipulated. Soon after the instrument was invented, it was recognized that in order to maximize the opportunities of AFM imaging in biology, various technological developments would be required to address certain limitations of the method. This has led to the creation of a range of new imaging modes, which continue to push the capabilities of the technique today. Here, we review the basic principles, advantages and limitations of the most common AFM bioimaging modes, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging. For each of these modes, we discuss recent experiments that highlight their unique capabilities. To reference this document use: http://resolver.tudelft.nl/uuid:712da3b2-8b23-4015-9f0b-dcebb7b62f37 DOI https://doi.org/10.1038/nnano.2017.45 Embargo date 2017-10-06 ISSN 1748-3387 Source Nature Nanotechnology, 12 (4), 295-307 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type review Rights © 2017 Yves F. Dufrêne, Toshio Ando, Ricardo Garcia, David Alsteens, David Martinez-Martin, A.H. Engel, Christoph Gerber, Daniel J. Müller Files PDF AFM_Bio_Manuscript_rev_20 ... 017_sm.pdf 1.08 MB Close viewer /islandora/object/uuid:712da3b2-8b23-4015-9f0b-dcebb7b62f37/datastream/OBJ/view