Print Email Facebook Twitter Diversity of cultivated aerobic poly-hydrolytic bacteria in saline alkaline soils Title Diversity of cultivated aerobic poly-hydrolytic bacteria in saline alkaline soils Author Sorokin, Dimitry Y. (TU Delft BT/Environmental Biotechnology; Russian Academy of Sciences) Kolganova, Tatiana V. (Russian Academy of Sciences) Khijniak, Tatiana V. (Russian Academy of Sciences) Jones, Brian E. (DuPont) Kublanov, Ilya V. (Russian Academy of Sciences; Immanuel Kant Baltic Federal University) Date 2017 Abstract Alkaline saline soils, known also as ''soda solonchaks'', represent a natural soda habitat which differs from soda lake sediments by higher aeration and lower humidity. The microbiology of soda soils, in contrast to the more intensively studied soda lakes, remains poorly explored. In this work we investigate the diversity of culturable aerobic haloalkalitolerant bacteria with various hydrolytic activities from soda soils at different locations in Central Asia, Africa, and North America. In total, 179 pure cultures were obtained by using media with various polymers at pH 10 and 0.6 M total Na+. According to the 16S rRNA gene sequence analysis, most of the isolates belonged to Firmicutes and Actinobacteria. Most isolates possessed multiple hydrolytic activities, including endoglucanase, xylanase, amylase and protease. The pH profiling of selected representatives of actinobacteria and endospore-forming bacteria showed, that the former were facultative alkaliphiles, while the latter were mostly obligate alkaliphiles. The hydrolases of selected representatives from both groups were active at a broad pH range from six to 11. Overall, this work demonstrates the presence of a rich hydrolytic bacterial community in soda soils which might be explored further for production of haloalkalistable hydrolases. Subject ActinobacteriaAerobicBacillusHaloalkaliphilicHydrolyticsSoda solonchak soils To reference this document use: http://resolver.tudelft.nl/uuid:740db18c-4276-4c1e-b74b-187fd68aba11 DOI https://doi.org/10.7717/peerj.3796 ISSN 2167-8359 Source PeerJ, 2017 (9) Part of collection Institutional Repository Document type journal article Rights © 2017 Dimitry Y. Sorokin, Tatiana V. Kolganova, Tatiana V. Khijniak, Brian E. Jones, Ilya V. Kublanov Files PDF peerj_3796.pdf 1.9 MB Close viewer /islandora/object/uuid:740db18c-4276-4c1e-b74b-187fd68aba11/datastream/OBJ/view