Print Email Facebook Twitter Nanowire arrays as cell force sensors to investigate adhesin-enhanced holdfast of single cell bacteria and biofilm stability Title Nanowire arrays as cell force sensors to investigate adhesin-enhanced holdfast of single cell bacteria and biofilm stability Author Sahoo, Prasana K. (University of Campinas) Janissen, R. (TU Delft BN/Nynke Dekker Lab; University of Campinas; Kavli institute of nanoscience Delft) Monteiro, Moniellen P. (University of Campinas) Cavalli, Alessandro (Eindhoven University of Technology) Murillo, Duber M. (University of Campinas) Merfa, Marcus V. (Agronomic Institute of Campinas) Cesar, Carlos L. (University of Campinas) Carvalho, Hernandes F. (University of Campinas) De Souza, Alessandra A. (Agronomic Institute of Campinas) Bakkers, E.P.A.M. (Eindhoven University of Technology) Cotta, Monica A. (University of Campinas) Date 2016 Abstract Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets for intervention and disease control. Here we employed InP nanowire arrays to dissect factors involved in the early stage biofilm formation of the phytopathogen Xylella fastidiosa. Ex vivo experiments demonstrate single-cell adhesion forces up to 45 nN, depending on the cell orientation with respect to the surface. Larger adhesion forces occur at the cell poles; secreted EPS layers and filaments provide additional mechanical support. Significant adhesion force enhancements were observed for single cells anchoring a biofilm and particularly on XadA1 adhesin-coated surfaces, evidencing molecular mechanisms developed by bacterial pathogens to create a stronger holdfast to specific host tissues. Subject adhesinBacterial biofilmcell adhesionforce sensornanowire arraysXylella fastidiosa To reference this document use: http://resolver.tudelft.nl/uuid:756fc5cd-389c-4fd9-b745-7a48d1f9c22c DOI https://doi.org/10.1021/acs.nanolett.6b01998 Embargo date 2017-06-23 ISSN 1530-6984 Source Nano Letters: a journal dedicated to nanoscience and nanotechnology, 16 (7), 4656-4664 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2016 Prasana K. Sahoo, R. Janissen, Moniellen P. Monteiro, Alessandro Cavalli, Duber M. Murillo, Marcus V. Merfa, Carlos L. Cesar, Hernandes F. Carvalho, Alessandra A. De Souza, E.P.A.M. Bakkers, Monica A. Cotta Files PDF NanoLetters_Post_Print.pdf 3.23 MB Close viewer /islandora/object/uuid:756fc5cd-389c-4fd9-b745-7a48d1f9c22c/datastream/OBJ/view