Print Email Facebook Twitter Photoenzymatic Hydroxylation of Ethylbenzene Catalyzed by Unspecific Peroxygenase Title Photoenzymatic Hydroxylation of Ethylbenzene Catalyzed by Unspecific Peroxygenase: Origin of Enzyme Inactivation and the Impact of Light Intensity and Temperature Author Burek, Bastien O. (Leibniz Universität; DECHEMA Research Institute) de Boer, Sabrina R. (DECHEMA Research Institute) Tieves, F. (TU Delft BT/Biocatalysis) Zhang, W. (TU Delft BT/Biocatalysis) van Schie, M.M.C.H. (TU Delft BT/Biocatalysis) Bormann, Sebastian (DECHEMA Research Institute) Alcalde, Miguel (Institute of Catalysis, CSIC, Madrid) Holtmann, Dirk (DECHEMA Research Institute) Hollmann, F. (TU Delft BT/Biocatalysis) Date 2019 Abstract Photoenzymatic cascades can be used for selective oxygenation of C−H-Bonds under mild conditions circumventing the hydrogen peroxide mediated peroxygenase inactivation via in situ H2O2 generation. Here, we report the “on demand” production of hydrogen peroxide via methanol assisted reduction of molecular oxygen using UV-illuminated titanium dioxide (Aeroxide P25) combined with the enantioselective hydroxylation of ethylbenzene to (R)-1-phenylethanole catalyzed by the Unspecific Peroxygenase from Agrocybe Aegerita. For the application of the system it is important to understand the influence of the reaction parameters to be able to optimize the system. Therefore, we systematically investigated product formation and enzyme inactivation as well as ROS formation (H2O2, .OH and .O2−) applying different light intensities and temperatures. As a result, Turnover Numbers up to 220 000, photonic efficiencies up to 13.6 % and production rates up to 0.9 mM h−1 were achieved. Subject enzyme inactivationhydrogen peroxidelight intensityperoxygenasesphotocatalysis To reference this document use: http://resolver.tudelft.nl/uuid:7da96064-c9fe-40b0-b97b-9e72f4707759 DOI https://doi.org/10.1002/cctc.201900610 Embargo date 2019-11-13 ISSN 1867-3880 Source ChemCatChem, 11 (13), 3093-3100 Bibliographical note Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public. Part of collection Institutional Repository Document type journal article Rights © 2019 Bastien O. Burek, Sabrina R. de Boer, F. Tieves, W. Zhang, M.M.C.H. van Schie, Sebastian Bormann, Miguel Alcalde, Dirk Holtmann, F. Hollmann, More Authors Files PDF artikel_Hollmann_Taverne_ ... ymatic.pdf 219.48 KB Close viewer /islandora/object/uuid:7da96064-c9fe-40b0-b97b-9e72f4707759/datastream/OBJ/view