Print Email Facebook Twitter 3D Tracking of Radioactive Particles in Small Animals Title 3D Tracking of Radioactive Particles in Small Animals Author Van Vliet, J.J.A. Contributor Kreuger, R. (mentor) Faculty Applied Sciences Department RD&M Date 2016-05-17 Abstract Fast and precise in vivo tracking of nanoparticles or single cells could provide better understanding of drug and stem cell delivery. Present SPECT with traditional collimators lack sensitivity to achieve sufficiently high frame rates. In this project a multi-slit SPECT system with stationary detectors was designed to track nanoparticles with a frame rate of 1000 frames per minute and a precision down to ~1 mm in a mouse-sized field of view (Ø25mmx100mm). The SPECT system used has three large gamma detectors in a stationary triangular configuration. A 198Au nanoparticle of 5 µm diameter can be activated as high as ~20 kBq and is inert in an aqueous solution, which makes it very suitable for in-vivo particle tracking. To track such a particle, a collimator system with four slits in the axial direction and one slit in the transversal plane was designed with a total sensitivity of 0.25%. Through each slit the particle gives a line-shaped response on a gamma camera. The position of the particle is found by solving the least squares solution of the intersection of the five planes defined by slits and the corresponding detector line responses. A particle tracking method with a Gaussian time window and interpolation between time frames with an insufficient number of counts is proposed. The design was experimentally evaluated by reconstructing the particle position at 448 positions in the field of view. Also, the trajectory was reconstructed of particle rotating with velocities between 6 mm/s and 20 mm/s. The position of a stationary 20 kBq source can be estimated with a precision of 0.1mm. A 20 kBq source moving at 6 mm/s could be tracked with a precision better than 2 mm at 1000 frames per minute. This is opening up possibilities for tracking single radioactive particles in small animals at high frame rates and a resolution close to a millimetre. Subject trackingradioactiveparticlesslitsslit-SPECTVECTorin-vivosmall animal To reference this document use: http://resolver.tudelft.nl/uuid:82ef2711-3a51-4f6b-92d2-ee7076c1b1b1 Part of collection Student theses Document type master thesis Rights (c) 2016 Van Vliet, J.J.A. Files PDF 3D Tracking of Radioactiv ... Vliet.pdf 1.96 MB Close viewer /islandora/object/uuid:82ef2711-3a51-4f6b-92d2-ee7076c1b1b1/datastream/OBJ/view