Print Email Facebook Twitter DNA nanopore translocation in glutamate solutions Title DNA nanopore translocation in glutamate solutions Author Plesa, C. Van Loo, N. Dekker, C. Faculty Applied Sciences Department Bionanoscience Date 2015-07-10 Abstract Nanopore experiments have traditionally been carried out with chloride-based solutions. Here we introduce silver/silver-glutamate-based electrochemistry as an alternative, and study the viscosity, conductivity, and nanopore translocation characteristics of potassium-, sodium-, and lithium-glutamate solutions. We show that it has a linear response at typical voltages and can be used to detect DNA translocations through a nanopore. The glutamate anion also acts as a redox-capable thickening agent, with high-viscosity solutions capable of slowing down the DNA translocation process by up to 11 times, with a corresponding 7 time reduction in signal. These results demonstrate that glutamate can replace chloride as the primary anion in nanopore resistive pulse sensing. Subject Gold for GoldOpen Access To reference this document use: http://resolver.tudelft.nl/uuid:83a50284-3ab2-4cb5-b0d1-620daf35f682 DOI https://doi.org/10.1039/C5NR02793D Publisher RSC Publishing ISSN 2040-3372 Source Nanoscale, 2015,7, 13605-13609 Part of collection Institutional Repository Document type journal article Rights (c) 2015 RSC Files PDF c5nr02793d.pdf 1.03 MB Close viewer /islandora/object/uuid:83a50284-3ab2-4cb5-b0d1-620daf35f682/datastream/OBJ/view