Analysis of the hybrid genomes of brewing yeasts

Analysis of the hybrid genomes of brewing yeasts

Bolat, I.

Pronk, J.T. (promotor)

Applied Sciences

Biotechnology

2016-01-06

One of the best guarded secrets of brewers is represented by the brewing yeast employed in beer fermentation, due to its profound impact upon the specific flavour profile of the final product. The current research tackles the genome diversity of lager brewing strains as well as their impact on important phenotypic traits. Furthermore, the development of a new recyclable dominant marker is described. This opened the way to unravel the molecular mechanism of cross-genome transcriptional regulation within a lager brewing strain. To prove the inherited genotypic and phenotypic advantages of the hybrid nature of bottom fermenting yeasts, an "artificial" hybrid was constructed between a haploid S.cerevisiae (italic) strain of the CEN.PK family and a haploid strain derived from the S. eubayanus (italic) type strain CBS12357, using mass mating. The heterosis (hybrid vigor) of the offspring proved the beneficial combination between the cold tolerance from one parent and the strong fermentative metabolism of sugars from the other parent. The research in this thesis brings some layers of transparency into the opaqueness of the genomes of lager brewing strains.

brewing
lager
yeast
genome

https://doi.org/10.4233/uuid:847a706c-2702-4de9-8e24-9738878c6689

Institutional Repository

doctoral thesis

(c) 2016 Bolat, I.