A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

Zhang, Z. (University of Chinese Academy of Sciences; Chinese Academy of Sciences)
Wu, Chenming (Tsinghua University)
Dai, C. (TU Delft Materials and Manufacturing)
Shi, Qingqing (Chinese Academy of Sciences)
Fang, G. (TU Delft Materials and Manufacturing; The University of Manchester)
Xie, Dongfang (Chinese Academy of Sciences)
Zhao, Xiangjie (University of Chinese Academy of Sciences; Chinese Academy of Sciences)
Liu, Yong Jin (Tsinghua University)
Wang, C.C. (TU Delft Materials and Manufacturing; The University of Manchester)
Wang, Xiu Jie (University of Chinese Academy of Sciences; Chinese Academy of Sciences)

2022

Despite the recent advances in artificial tissue and organ engineering, how to generate large size viable and functional complex organs still remains as a grand challenge for regenerative medicine. Three-dimensional bioprinting has demonstrated its advantages as one of the major methods in fabricating simple tissues, yet it still faces difficulties to generate vasculatures and preserve cell functions in complex organ production. Here, we overcome the limitations of conventional bioprinting systems by converting a six degree-of-freedom robotic arm into a bioprinter, therefore enables cell printing on 3D complex-shaped vascular scaffolds from all directions. We also developed an oil bath-based cell printing method to better preserve cell natural functions after printing. Together with a self-designed bioreactor and a repeated print-and-culture strategy, our bioprinting system is capable to generate vascularized, contractible, and long-term survived cardiac tissues. Such bioprinting strategy mimics the in vivo organ development process and presents a promising solution for in vitro fabrication of complex organs.

3D bioprinting
Artificial organ engineering
Cardiac tissue fabrication
Print-and-culture
Six degree-of-freedom robot

http://resolver.tudelft.nl/uuid:876aa936-ad3f-479b-a30a-0208e67d9894

https://doi.org/10.1016/j.bioactmat.2022.02.009

2452-199X

Bioactive Materials, 18, 138-150

Institutional Repository

journal article

© 2022 Z. Zhang, Chenming Wu, C. Dai, Qingqing Shi, G. Fang, Dongfang Xie, Xiangjie Zhao, Yong Jin Liu, C.C. Wang, Xiu Jie Wang