Print Email Facebook Twitter Aerobic growth physiology of Saccharomyces cerevisiae on sucrose is strain-dependent Title Aerobic growth physiology of Saccharomyces cerevisiae on sucrose is strain-dependent Author Soares Rodrigues, C.I. (TU Delft BT/Industriele Microbiologie) Wahl, S.A. (TU Delft BT/Industriele Microbiologie) Gombert, Andreas K. (University of Campinas) Date 2021 Abstract Present knowledge on the quantitative aerobic physiology of the yeast Saccharomyces cerevisiae during growth on sucrose as sole carbon and energy source is limited to either adapted cells or to the model laboratory strain CEN.PK113-7D. To broaden our understanding of this matter and open novel opportunities for sucrose-based biotechnological processes, we characterized three strains, with distinct backgrounds, during aerobic batch bioreactor cultivations. Our results reveal that sucrose metabolism in S. cerevisiae is a strain-specific trait. Each strain displayed distinct extracellular hexose concentrations and invertase activity profiles. Especially, the inferior maximum specific growth rate (0.21 h-1) of the CEN.PK113-7D strain, with respect to that of strains UFMG-CM-Y259 (0.37 h-1) and JP1 (0.32 h-1), could be associated to its low invertase activity (0.04-0.09 U/mgDM). Moreover, comparative experiments with glucose or fructose alone, or in combination, suggest mixed mechanisms of sucrose utilization by the industrial strain JP1, and points out the remarkable ability of the wild isolate UFMG-CM-259 to grow faster on sucrose than on glucose in a well-controlled cultivation system. This work hints to a series of metabolic traits that can be exploited to increase sucrose catabolic rates and bioprocess efficiency. Subject Saccharomyces cerevisiaeinvertasesucroseyeast physiology To reference this document use: http://resolver.tudelft.nl/uuid:8a307290-e0ed-49b2-b2a1-5bdf690affeb DOI https://doi.org/10.1093/femsyr/foab021 Embargo date 2022-04-07 ISSN 1567-1356 Source FEMS Yeast Research, 21 (3) Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2021 C.I. Soares Rodrigues, S.A. Wahl, Andreas K. Gombert Files PDF file_2_.pdf 5.43 MB Close viewer /islandora/object/uuid:8a307290-e0ed-49b2-b2a1-5bdf690affeb/datastream/OBJ/view