Studying genomic processes at the single-molecule level: introducing the tools and applications

Dulin, D.; Lipfert, J.; Moolman, M.C.; Dekker, N.H.

doi:10.1038/nrg3316

Studying genomic processes at the single-molecule level

Title

Studying genomic processes at the single-molecule level: introducing the tools and applications

Author

Dulin, D. (TU Delft BN/Nynke Dekker Lab)
Lipfert, J. (TU Delft BN/Nynke Dekker Lab)
Moolman, M.C. (TU Delft BN/Nynke Dekker Lab)
Dekker, N.H. (TU Delft BN/Nynke Dekker Lab)

Date

2013

Abstract

To understand genomic processes such as transcription, translation or splicing, we need to be able to study their spatial and temporal organization at the molecular level. Single-molecule approaches provide this opportunity, allowing researchers to monitor molecular conformations, interactions or diffusion quantitatively and in real time in purified systems and in the context of the living cell. This Review introduces the types of application of single-molecule approaches that can enhance our understanding of genome function.

Subject

Gene expression
Genomics
Microbial genetics
Technology

To reference this document use:

http://resolver.tudelft.nl/uuid:8c6de115-9c4e-4e96-8f80-197db0a3f545

DOI

https://doi.org/10.1038/nrg3316

ISSN

1471-0056

Source

Nature Reviews. Genetics, 14 (1), 9-22

Bibliographical note

Accepted Author Manuscript