Print Email Facebook Twitter The Oxygen Dilemma Title The Oxygen Dilemma: A Severe Challenge for the Application of Monooxygenases? Author Holtmann, Dirk (DECHEMA Research Institute) Hollmann, F. (TU Delft BT/Biocatalysis) Date 2016-08-03 Abstract Monooxygenases are promising catalysts because they in principle enable the organic chemist to perform highly selective oxyfunctionalisation reactions that are otherwise difficult to achieve. For this, monooxygenases require reducing equivalents, to allow reductive activation of molecular oxygen at the enzymes' active sites. However, these reducing equivalents are often delivered to O2 either directly or via a reduced intermediate (uncoupling), yielding hazardous reactive oxygen species and wasting valuable reducing equivalents. The oxygen dilemma arises from monooxygenases' dependency on O2 and the undesired uncoupling reaction. With this contribution we hope to generate a general awareness of the oxygen dilemma and to discuss its nature and some promising solutions. Subject biocatalysismonooxygenasesoxidoreductasesoxyfunctionalizationuncoupling To reference this document use: http://resolver.tudelft.nl/uuid:9009f954-1b92-4a2e-841b-af958468f443 DOI https://doi.org/10.1002/cbic.201600176 ISSN 1439-4227 Source ChemBioChem: a European journal of chemical biology, 17 (15), 1391-1398 Part of collection Institutional Repository Document type journal article Rights © 2016 Dirk Holtmann, F. Hollmann Files PDF cbic.201600176.pdf 1.47 MB Close viewer /islandora/object/uuid:9009f954-1b92-4a2e-841b-af958468f443/datastream/OBJ/view