Print Email Facebook Twitter Periodic Switching in a Recombinase-Based Molecular Circuit Title Periodic Switching in a Recombinase-Based Molecular Circuit Author Samaniego, Christian Cuba (Arizona State University; Massachusetts Institute of Technology) Giordano, G. (TU Delft Team Tamas Keviczky) Franco, Elisa (University of California) Date 2020 Abstract An important challenge in synthetic biology is the construction of periodic circuits with tunable and predictable period. We propose a general architecture, based on the use of recombinase proteins and negative feedback, to build a molecular device for periodic switching between two distinct regimes; the switching rule depends on known concentration thresholds for some circuit components. We analytically characterize the threshold values for which a periodic orbit is guaranteed to exist and attract all trajectories with initial conditions within an invariant set, and we provide expressions for period and amplitude. We describe two distinct biological realizations of the recombinase architecture, and show their capacity to exhibit periodic behaviors via extensive numerical simulations. Subject Biomolecular systemssystems biology To reference this document use: http://resolver.tudelft.nl/uuid:911267e9-c8c8-4229-a9e6-b1479e0c350c DOI https://doi.org/10.1109/LCSYS.2019.2923271 ISSN 2475-1456 Source IEEE Control Systems Letters, 4 (1), 241-246 Bibliographical note Accepted Author Manuscript Part of collection Institutional Repository Document type journal article Rights © 2020 Christian Cuba Samaniego, G. Giordano, Elisa Franco Files PDF 2020LCSS_BCCG.pdf 458.69 KB Close viewer /islandora/object/uuid:911267e9-c8c8-4229-a9e6-b1479e0c350c/datastream/OBJ/view