Comparison of media for enumeration of Clostridium perfringens from foods

article
2003
Jong, A.E.I. de

Eijhusen, G.P.

Brouwer-Post, E.J.F.

Grand, M.

Johansson, T.

Kärkkäinen, T.

Marugg, J.

Veld, P.H. in 't

Warmerdam, F.H.M.

Wörner, G.

Zicavo, A.

Rombouts, F.M.

Beumer, R.R.
Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens (SFP) agar, sulfite cycloserine azide (SCA), differential clostridial agar (DCA), and oleandomycin polymyxin sulfadiazine perfringens (OPSP) agar] were compared in a prestudy, of which four (IS, TSC, SCA, and DCA) were selected for an international collaborative trial. Recovery of 15 pure strains was tested in the prestudy and recovery of one strain from foodstuffs was tested in the collaborative trial. Results from the prestudy did reveal statistical difference of the media but recoveries on all media were within the microbiological limits (±30%) of IS, which was set as a reference medium. Recoveries on the media tested in the collaborative trial were statistically different as well, but these differences were of no microbiological-analytical relevance. Food matrices did not affect the recovery of C. perfringens in general. DCA and SCA, in particular, are labor-intensive to prepare and DCA frequently failed to produce black colonies; gray colonies were quite common. Since IS medium is nonselective, it was concluded that TSC was the most favorable medium for the enumeration of C. perfringens from foods. © 2003 Elsevier Science B.V. All rights reserved.
Topics
BiotechnologyClostridium perfringensEnumerationMediaAgarAzideCycloserineIronOleandomycinPolymyxinSugarSulfadiazineSulfiteAgar mediumBacterial countBacterial strainBacterium colonyBacterium contaminationBacterium isolationClostridium perfringensComparative studyControlled studyCulture mediumFoodFood analysisFood contaminationMicrobiological examinationNonhumanPriority journalAnimalsClostridium perfringensColony Count, MicrobialCulture MediaFood MicrobiologyMilkPilot ProjectsSpores, BacterialClostridiumClostridium perfringens
TNO Identifier
237273
Repository link
https://resolver.tno.nl/uuid:94486972-e13b-4e46-86a1-d7bcbb29c064
ISSN
01677012
Source
Journal of Microbiological Methods, 54(3), pp. 359-366.
Pages
359-366
Files
To receive the publication files, please send an e-mail request to TNO Repository.