Print Email Facebook Twitter Genome duplication and mutations in ACE2 cause multicellular, fast-sedimenting phenotypes in evolved Saccharomyces cerevisiae Title Genome duplication and mutations in ACE2 cause multicellular, fast-sedimenting phenotypes in evolved Saccharomyces cerevisiae Author Oud, B. Guadalupe-Medina, V. Nijkamp, J.F. De Ridder, D. Pronk, J.T. Van Maris, A.J.A. Daran, J.G. Faculty Applied Sciences Department BT/Biotechnology Date 2013-10-21 Abstract Laboratory evolution of the yeast Saccharomyces cerevisiae in bioreactor batch cultures yielded variants that grow as multicellular, fast-sedimenting clusters. Knowledge of the molecular basis of this phenomenon may contribute to the understanding of natural evolution of multicellularity and to manipulating cell sedimentation in laboratory and industrial applications of S. cerevisiae. Multicellular, fast-sedimenting lineages obtained from a haploid S. cerevisiae strain in two independent evolution experiments were analyzed by whole genome resequencing. The two evolved cell lines showed different frameshift mutations in a stretch of eight adenosines in ACE2, which encodes a transcriptional regulator involved in cell cycle control and motherdaughter cell separation. Introduction of the two ace2 mutant alleles into the haploid parental strain led to slow-sedimenting cell clusters that consisted of just a few cells, thus representing only a partial reconstruction of the evolved phenotype. In addition to single-nucleotide mutations, a whole-genome duplication event had occurred in both evolved multicellular strains. Construction of a diploid reference strain with two mutant ace2 alleles led to complete reconstruction of the multicellular-fast sedimenting phenotype. This study shows that whole-genome duplication and a frameshift mutation in ACE2 are sufficient to generate a fast-sedimenting, multicellular phenotype in S. cerevisiae. The nature of the ace2 mutations and their occurrence in two independent evolution experiments encompassing fewer than 500 generations of selective growth suggest that switching between unicellular and multicellular phenotypes may be relevant for competitiveness of S. cerevisiae in natural environments. Subject whole genome sequencingreverse engineering To reference this document use: http://resolver.tudelft.nl/uuid:974d45fc-0aa1-4bbf-be83-bb63b3dc5f4b DOI https://doi.org/10.1073/pnas.1305949110 Publisher National Academy of Sciences ISSN 1091-6490 Source Proceedings of the National Academy of Sciences, 110 (45), 2013 Part of collection Institutional Repository Document type journal article Rights (c) 2013 The Author(s) Files PDF Daran 2013.pdf 1.03 MB Close viewer /islandora/object/uuid:974d45fc-0aa1-4bbf-be83-bb63b3dc5f4b/datastream/OBJ/view