Print Email Facebook Twitter The interplay between polymerase organization and nucleosome occupancy along DNA Title The interplay between polymerase organization and nucleosome occupancy along DNA: How dynamic roadblocks on the DNA induce the formation of RNA polymerase pelotons Author van den Berg, A.A. (TU Delft BN/Martin Depken Lab) Contributor Dekker, N.H. (promotor) Depken, S.M. (copromotor) Degree granting institution Delft University of Technology Date 2017-09-29 Abstract During transcription RNA polymerase (RNAP) moves along a DNA molecule to copy the information on the DNA to an RNA molecule. Many textbook pictures show an RNAP sliding along empty DNA, but in reality it is crowded on the DNA and RNAP competes for space with many proteins such as other RNAP’s and histones. Coverage of DNA by histones is essential for DNA protection and signaling. However, during transcription RNAP evicts histones, which then rebind quickly or are replaced by other proteins. How does crowding of RNAP and histones on the DNA affects transcription dynamics on the one hand, and how does transcription activity change the density and exchange of histones along the DNA on the other hand? Those are the central questions of this thesis. Subject Transcriptiontranscriptional burstsTASEPCrowdingNucleosomesBus route model To reference this document use: https://doi.org/10.4233/uuid:993e98ca-3c91-4591-9fbf-26bd6eea2354 ISBN 978-90-8593-308-3 Bibliographical note Casimir PhD series, Delft-Leiden 2017-24 Part of collection Institutional Repository Document type doctoral thesis Rights © 2017 A.A. van den Berg Files PDF Dissertation_AAvdBerg_Rep ... sitory.pdf 6.3 MB Close viewer /islandora/object/uuid:993e98ca-3c91-4591-9fbf-26bd6eea2354/datastream/OBJ/view