Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

Li, D.D.U.
Arlt, J.
Tyndall, D.
Walker, R.
Richardson, J.
Stoppa, D.
Charbon, E.
Henderson, R.K.

Electrical Engineering, Mathematics and Computer Science

Microelectronics

2011-09-15

A high-speed and hardware-only algorithm using a center of mass method has been proposed for single-detector fluorescence lifetime sensing applications. This algorithm is now implemented on a field programmable gate array to provide fast lifetime estimates from a 32 × 32 low dark count 0.13 ?m complementary metaloxide-semiconductor single-photon avalanche diode (SPAD) plus time-to-digital converter array. A simple look-up table is included to enhance the lifetime resolvability range and photon economics, making it comparable to the commonly used least-square method and maximum likelihood estimation based software. To demonstrate its performance, a widefield microscope was adapted to accommodate the SPAD array and image different test samples. Fluorescence lifetime imaging microscopy on fluorescent beads in Rhodamine 6G at a frame rate of 50 fps is also shown.

fluorescence lifetime imaging microscopy
time-resolved imaging
photon counting
complementary metal-oxidesemiconductor
single-photon avalanche diode
center-of-mass

http://resolver.tudelft.nl/uuid:9aa3b277-1b45-4b0c-b9be-5f66dc24697a

https://doi.org/10.1117/1.3625288

Society of Photo-optical Instrumentation Engineers (SPIE)

1083-3668

https://doi.org/10.1117/1.3625288

Journal of Biomedical Optics, 16 (9), 2011

Institutional Repository

journal article

(c) 2011 The Author(s)
SPIE