Print Email Facebook Twitter Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus Title Production of α-1,3-L-arabinofuranosidase active on substituted xylan does not improve compost degradation by Agaricus bisporus Author Vos, A.M. (TU Delft BT/Industriele Microbiologie; Universiteit Utrecht) Jurak, Edita (Wageningen University & Research; Aalto University) de Gijsel, Peter (Wageningen University & Research) Ohm, Robin A. (Universiteit Utrecht) Henrissat, Bernard (Aix Marseille Université) Lugones, Luis G. (Universiteit Utrecht) Kabel, Mirjam A. (Wageningen University & Research) Wösten, Han A.B. (Universiteit Utrecht) Date 2018-07-24 Abstract Agaricus bisporus consumes carbohydrates contained in wheat straw based compost used for commercial mushroom production. Double substituted arabinoxylan is part of the ~40% of the compost polysaccharides that are not degraded by A. bisporus during its growth and development. Genes encoding α-1,3-L-arabinofuranosidase (AXHd3) enzymes that act on xylosyl residues doubly substituted with arabinosyl residues are absent in this mushroom forming fungus. Here, the AXHd3 encoding hgh43 gene of Humicola insolens was expressed in A. bisporus with the aim to improve its substrate utilization and mushroom yield. Transformants secreted active AXHd3 in compost as shown by the degradation of double substituted arabinoxylan oligomers in an in vitro assay. However, carbohydrate composition and degree of arabinosyl substitution of arabinoxylans were not affected in compost possibly due to inaccessibility of the doubly substituted xylosyl residues. To reference this document use: http://resolver.tudelft.nl/uuid:9abe94a0-591e-45b0-9b2a-2e18b4c49f28 DOI https://doi.org/10.1371/journal.pone.0201090 ISSN 1932-6203 Source PLoS ONE, 13 (7) Part of collection Institutional Repository Document type journal article Rights © 2018 A.M. Vos, Edita Jurak, Peter de Gijsel, Robin A. Ohm, Bernard Henrissat, Luis G. Lugones, Mirjam A. Kabel, Han A.B. Wösten Files PDF journal.pone.0201090.pdf 936.11 KB Close viewer /islandora/object/uuid:9abe94a0-591e-45b0-9b2a-2e18b4c49f28/datastream/OBJ/view